{"id":153,"date":"2021-12-06T12:16:43","date_gmt":"2021-12-06T11:16:43","guid":{"rendered":"http:\/\/wordpress.p604513.webspaceconfig.de\/research\/projektbereich-b\/"},"modified":"2023-11-07T12:16:27","modified_gmt":"2023-11-07T11:16:27","slug":"project-area-b","status":"publish","type":"page","link":"https:\/\/www.loewe-druid.de\/en\/research\/project-area-b\/","title":{"rendered":"Project area B"},"content":{"rendered":"\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-av_one_fourth-003d50325157ae76c6cf2b217f70282b\">\n.flex_column.av-av_one_fourth-003d50325157ae76c6cf2b217f70282b{\nborder-radius:0px 0px 0px 0px;\npadding:0px 0px 0px 0px;\n}\n<\/style>\n<div  class='flex_column av-av_one_fourth-003d50325157ae76c6cf2b217f70282b av_one_fourth  avia-builder-el-0  el_before_av_three_fourth  avia-builder-el-first  rahmen-headline-projects first flex_column_div av-zero-column-padding  '     ><style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-kzqx28qa-567ac1969f707645ff557f4cfec51c98\">\n#top .av-special-heading.av-kzqx28qa-567ac1969f707645ff557f4cfec51c98{\npadding-bottom:10px;\n}\nbody .av-special-heading.av-kzqx28qa-567ac1969f707645ff557f4cfec51c98 .av-special-heading-tag .heading-char{\nfont-size:25px;\n}\n.av-special-heading.av-kzqx28qa-567ac1969f707645ff557f4cfec51c98 .av-subheading{\nfont-size:15px;\n}\n<\/style>\n<div  class='av-special-heading av-kzqx28qa-567ac1969f707645ff557f4cfec51c98 av-special-heading-h1 blockquote modern-quote  avia-builder-el-1  avia-builder-el-no-sibling '><h1 class='av-special-heading-tag '  itemprop=\"headline\"  >Project area B:<\/h1><div class=\"special-heading-border\"><div class=\"special-heading-inner-border\"><\/div><\/div><\/div><\/div>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-av_three_fourth-74b9a86a12f903134a10607d86902188\">\n.flex_column.av-av_three_fourth-74b9a86a12f903134a10607d86902188{\nborder-radius:0px 0px 0px 0px;\npadding:0px 0px 0px 0px;\n}\n<\/style>\n<div  class='flex_column av-av_three_fourth-74b9a86a12f903134a10607d86902188 av_three_fourth  avia-builder-el-2  el_after_av_one_fourth  avia-builder-el-last  rahmen-accordion-projects border-left flex_column_div av-zero-column-padding  '     ><div class=\"togglecontainer toggle_close_all  avia-builder-el-5  avia-builder-el-no-sibling  enable_toggles projekte_toggle\"><section class=\"av_toggle_section\" >    <div role=\"tablist\" class=\"single_toggle\" data-tags=\"{Alle}\">        <p data-fake-id=\"#toggle-id-1\" class=\"toggler av-inherit-border-color\" role=\"tab\" tabindex=\"0\" aria-controls=\"1toggle-id-container\"><span class=\"project_nr\"> B1<\/span><span class=\"project_title\">Dengue and Zika virus, design of inhibitors of NS3\/NS2B serine protease.<\/span><br><span class=\"project_names\">Wibke Diederich, Peter Kolb<\/span><span class=\"toggle_icon\">        <span class=\"vert_icon\"><\/span><span class=\"hor_icon\"><\/span><\/span><\/p>        <div id=\"toggle-id-1-container\" class=\"toggle_wrap\">            <div class=\"toggle_content invers-color invers-color  av-inherit-border-color flex-container\">              <div class=\"projekt-names one_three\"><div class=\"project-name\"><div class=\"flex-container\"><div class=\"half\"><span class=\"principal\">Principal Investigator:<\/span><\/div><div class=\"half\"><a href=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/wibke-diederich.jpg\"><img decoding=\"async\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/wibke-diederich.jpg\" \/><\/a><\/div><\/div><p class=\"person_name\"><strong>Prof. Dr. Wibke Diederich<\/strong><\/p><p><p>Institut f\u00fcr Pharmazeutische Chemie<br \/>\nZentrum f\u00fcr Tumor- und Immunbiologie<br \/>\nPhilipps-Universit\u00e4t Marburg<br \/>\nHans-Meerwein-Stra\u00dfe 3<br \/>\n35043 Marburg<br \/>\nTel.: +49 (0)6421-28 25810<br \/>\nFax: +49 (0)6421-28 26254<br \/>\nE-Mail: <a class=\"mail\" href=\"mailto:wibke.diederich@staff.uni-marburg.de\">wibke.diederich(at)staff.uni-marburg(dot)de<\/a><\/p>\n<p><a href=\"https:\/\/www.uni-marburg.de\/de\/fb16\/ipc\/ak-diederich\" target=\"_blank\" rel=\"noopener\">Homepage<\/a><\/p>\n<\/p><\/div><hr><div class=\"project-name\"><div class=\"flex-container\"><div class=\"half\"><span class=\"principal\">Principal Investigator:<\/span><\/div><div class=\"half\"><a href=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/peter-kolb.jpg\"><img decoding=\"async\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/peter-kolb.jpg\" \/><\/a><\/div><\/div><p class=\"person_name\"><strong>Prof. Dr. Peter Kolb<\/strong><\/p><p><p>Institut f\u00fcr Pharmazeutische Chemie<br \/>\nPhilipps-Universit\u00e4t Marburg<br \/>\nMarbacher Weg 8<br \/>\n35032 Marburg<br \/>\nTel.: +49 (0)6421-28 25908<br \/>\nFax: +49 (0)6421-28 26652<br \/>\nE-Mail: <a class=\"mail\" href=\"mailto:peter.kolb@uni-marburg.de\">peter.kolb(at)uni-marburg(dot)de<\/a><\/p>\n<p><a href=\"https:\/\/www.uni-marburg.de\/en\/fb16\/ipc\/kolb-group\" target=\"_blank\" rel=\"noopener\">Homepage<\/a><\/p>\n<\/p><\/div><hr><\/div>\t\t\t <div class=\"project-content two_three border-left\"><p><strong>Project description:<\/strong><\/p>\n<p>Infections with the dengue virus have reached a new high in recent years, with around 50-100 million new infections per year. In the vast majority of cases, the infection progresses with mild, flu-like symptoms, but a small percentage of those affected, often children, develop hemorrhagic fever, which is fatal if severe. Although a vaccine is now available, since this is only approved for a very limited group of people, the development of agents that efficiently suppress the multiplication of the virus is essential. Our work focuses on the virus&#8217;s own serine protease NS3\/NS2B, which cleaves the viral precursor protein into functional proteins and is essential for the maturation of the virus.<\/p>\n<div id=\"attachment_499\" style=\"width: 310px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-499\" class=\"wp-image-499 size-medium\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/\/b01-grafik-01-300x261.png\" alt=\"\" width=\"300\" height=\"261\" srcset=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/b01-grafik-01-300x261.png 300w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/b01-grafik-01.png 437w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><p id=\"caption-attachment-499\" class=\"wp-caption-text\">Abb. B1. Kristallstruktur der NS3\/NS2B Protease DENV 3.1<\/p><\/div>\n<p><strong>Scientific goal: <\/strong><\/p>\n<p>The aim of the project is to further develop allosteric inhibitors of the viral serine protease NS3\/NS2B, not only with respect to their affinity, but also with respect to their pharmacokinetic properties and toxicity (hit-to-lead development) using a combined approach of computer-aided design, synthesis, biological assays and crystal structure analysis. In addition, inhibitors of the related Zika protease will be developed based on the knowledge gained.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>DRUID Collaboration partners<\/strong><strong>:<\/strong><\/p>\n<p>A1 Becker, A2 Gr\u00fcnweller, B3 Rahlfs\/Kolb\/van Zandbergen, XY Herker, E6 Schiffmann\/Laux<\/p>\n<hr \/>\n<p>References B1: <strong>[1] <\/strong>Noble <em>et al. <\/em><strong>(2012) <em>J Virol <\/em><\/strong>86(1):438-446; *<strong>[2] <\/strong>Chevillard <em>et al. <\/em><strong>(2015), <em>J Chem Inf Model <\/em><\/strong>55(9):1824-1835; *<strong>[3] <\/strong>Chevillard <em>et al. <\/em><strong>(2018) <em>J Med Chem<\/em><\/strong> 61(3):1118-1129<\/p>\n<\/div>            <\/div>        <\/div>    <\/div><\/section><section class=\"av_toggle_section\" >    <div role=\"tablist\" class=\"single_toggle\" data-tags=\"{Alle}\">        <p data-fake-id=\"#toggle-id-1\" class=\"toggler av-inherit-border-color\" role=\"tab\" tabindex=\"0\" aria-controls=\"1toggle-id-container\"><span class=\"project_nr\"> B2<\/span><span class=\"project_title\">Protein de-ADP-ribosylation und NMPylation activities as potential therapeutic targets against coronaviruses<\/span><br><span class=\"project_names\">John Ziebuhr<\/span><span class=\"toggle_icon\">        <span class=\"vert_icon\"><\/span><span class=\"hor_icon\"><\/span><\/span><\/p>        <div id=\"toggle-id-1-container\" class=\"toggle_wrap\">            <div class=\"toggle_content invers-color invers-color  av-inherit-border-color flex-container\">              <div class=\"projekt-names one_three\"><div class=\"project-name\"><div class=\"flex-container\"><div class=\"half\"><span class=\"principal\">Principal Investigator:<\/span><\/div><div class=\"half\"><a href=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/john-ziebuhr.jpg\"><img decoding=\"async\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/john-ziebuhr.jpg\" \/><\/a><\/div><\/div><p class=\"person_name\"><strong>Prof. Dr. John Ziebuhr<\/strong><\/p><p><p>Institut f\u00fcr Medizinische Virologie<br \/>\nBiomedizinisches Forschungszentrum<br \/>\nSeltersberg (BFS)<br \/>\nJustus-Liebig-Universit\u00e4t Gie\u00dfen<br \/>\nSchubertstra\u00dfe 81<br \/>\n35392 Gie\u00dfen<br \/>\nTel.: +49 (0)641-99 41200<br \/>\nFax: +49 (0)641-99 41209<br \/>\nE-Mail: <a class=\"mail\" href=\"mailto:John.Ziebuhr@viro.med.uni-giessen.de\">John.Ziebuhr(at)viro.med.uni-giessen(dot)de<\/a><\/p>\n<\/p><\/div><hr><\/div>\t\t\t <div class=\"project-content two_three border-left\"><p><strong>Project description:<\/strong><\/p>\n<p>Coronaviruses are important human and animal pathogens. They are mainly associated with respiratory and intestinal infections and have significant zoonotic potential, resulting in several outbreaks of severe respiratory infections in humans over the past 2 decades including the SARS-CoV-2 pandemic starting in 2019. Therapeutic options to treat severe forms of COVID-19 and other coronavirus infections are very limited, indicating a high priority for the development of novel antiviral drugs. To address this need, project B2 focuses on two coronaviral proteins that are conserved among all coronaviruses: the coronavirus macrodomain (macD) in nonstructural protein 3 (nsp3) and the recently discovered nucleotidyltransferase (NiRAN) which is linked to the viral RNA-dependent RNA polymerase domain in nsp12 and was recently shown to be essential for coronavirus replication.<\/p>\n<div id=\"attachment_505\" style=\"width: 310px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-505\" class=\"wp-image-505 size-medium\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/\/b02-grafik-01-300x23.png\" alt=\"\" width=\"300\" height=\"23\" srcset=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/b02-grafik-01-300x23.png 300w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/b02-grafik-01-1030x78.png 1030w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/b02-grafik-01-768x58.png 768w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/b02-grafik-01-705x53.png 705w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/b02-grafik-01.png 1361w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><p id=\"caption-attachment-505\" class=\"wp-caption-text\">Coronavirus replicase polyprotein 1ab. Proteolytic processing by two or three viral proteases (PL, 3CL) results in the release of up to 16 nonstructural proteins (nsp) with numerous enzymatic and other functions.<\/p><\/div>\n<p><strong>Scientific goal: <\/strong><\/p>\n<p>The project aims to comprehensively characterize the biochemical properties of two coronavirus proteins\/enzymes (macD, NiRAN) and their functions in the viral replication cycle using appropriate cell culture systems. Based on the conserved substrate specificity of the NiRAN domain, HTS assays will be developed and used to identify potential inhibitors.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>DRUID Collaboration partners<\/strong><strong>:<\/strong><\/p>\n<p>A2 Gr\u00fcnweller, A3 Weber, C5 Kraiczy, E3 Rahlfs\/Przyborski<\/p>\n<hr \/>\n<p>References B2: <strong>1.\u00a0<\/strong>*Slanina <em>et al. <\/em><strong>(2021) <em>Proc Natl Acad Sci USA <\/em><\/strong>118:\u00a0e2022310118 <strong>2. <\/strong>*Krichel <em>et al. <\/em><strong>(2021) <em>Sci. Adv. <\/em><\/strong>7:\u00a0eabf1004<em> <strong>\u00a0<\/strong><\/em><strong>3.<\/strong>\u00a0*M\u00fcller\u00a0<em>et al.<\/em>\u00a0<strong>(2021)<\/strong>\u00a0<strong><em>Antiviral Res<\/em>\u00a0<\/strong>175: 1004706<strong>\u00a04.<\/strong>\u00a0*Snijder\u00a0<em>et al.<\/em>\u00a0<strong>(2016)\u00a0Adv Virus Res <\/strong>96: 59-126 <strong>5.<\/strong>\u00a0*Putics\u00a0<em>et al.<\/em>\u00a0<strong>(2005)<\/strong>\u00a0<strong><em>J Virol\u00a0<\/em><\/strong>79:\u00a012721-31<em>.<\/em><\/p>\n<\/div>            <\/div>        <\/div>    <\/div><\/section><section class=\"av_toggle_section\" >    <div role=\"tablist\" class=\"single_toggle\" data-tags=\"{Alle}\">        <p data-fake-id=\"#toggle-id-1\" class=\"toggler av-inherit-border-color\" role=\"tab\" tabindex=\"0\" aria-controls=\"1toggle-id-container\"><span class=\"project_nr\"> B3<\/span><span class=\"project_title\">NAD(P)H-dependent metabolic pathways as targets for new anti-infective agents<\/span><br><span class=\"project_names\">Stefan Rahlfs, Ger van Zandbergen, Peter Kolb<\/span><span class=\"toggle_icon\">        <span class=\"vert_icon\"><\/span><span class=\"hor_icon\"><\/span><\/span><\/p>        <div id=\"toggle-id-1-container\" class=\"toggle_wrap\">            <div class=\"toggle_content invers-color invers-color  av-inherit-border-color flex-container\">              <div class=\"projekt-names one_three\"><div class=\"project-name\"><div class=\"flex-container\"><div class=\"half\"><span class=\"principal\">Principal Investigator:<\/span><\/div><div class=\"half\"><a href=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/stefan-rahlfs.jpg\"><img decoding=\"async\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/stefan-rahlfs.jpg\" \/><\/a><\/div><\/div><p class=\"person_name\"><strong>Dr. Stefan Rahlfs<\/strong><\/p><p><p>Biochemie und Molekularbiologie<br \/>\nJustus-Liebig-Universit\u00e4t Gie\u00dfen<br \/>\nHeinrich-Buff-Ring 26-32<br \/>\n35390 Gie\u00dfen<br \/>\nTel.: +49 (0)641-99 39117<br \/>\nE-Mail: <a class=\"mail\" href=\"mailto:stefan.rahlfs@ernaehrung.uni-giessen.de\">stefan.rahlfs(at)ernaehrung.uni-giessen(dot)de<\/a><\/p>\n<\/p><\/div><hr><div class=\"project-name\"><div class=\"flex-container\"><div class=\"half\"><span class=\"principal\">Principal Investigator:<\/span><\/div><div class=\"half\"><a href=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/ger-van-zandbergen.jpg\"><img decoding=\"async\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/ger-van-zandbergen.jpg\" \/><\/a><\/div><\/div><p class=\"person_name\"><strong>Prof. Dr. Ger van Zandbergen<\/strong><\/p><p><p>Abteilung Immunologie<br \/>\nPaul-Ehrlich-Institut<br \/>\nPaul-Ehrlich-Str. 51-59<br \/>\n63225 Langen<br \/>\nTel.: +49 (0)6103-77 2005<br \/>\nE-Mail: <a class=\"mail\" href=\"mailto:Ger.Zandbergen@pei.de\">Ger.Zandbergen(at)pei(dot)de<\/a><\/p>\n<\/p><\/div><hr><div class=\"project-name\"><div class=\"flex-container\"><div class=\"half\"><span class=\"principal\">Principal Investigator:<\/span><\/div><div class=\"half\"><a href=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/peter-kolb.jpg\"><img decoding=\"async\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/peter-kolb.jpg\" \/><\/a><\/div><\/div><p class=\"person_name\"><strong>Prof. Dr. Peter Kolb<\/strong><\/p><p><p>Institut f\u00fcr Pharmazeutische Chemie<br \/>\nPhilipps-Universit\u00e4t Marburg<br \/>\nMarbacher Weg 8<br \/>\n35032 Marburg<br \/>\nTel.: +49 (0)6421-28 25908<br \/>\nFax: +49 (0)6421-28 26652<br \/>\nE-Mail: <a class=\"mail\" href=\"mailto:peter.kolb@uni-marburg.de\">peter.kolb(at)uni-marburg(dot)de<\/a><\/p>\n<p><a href=\"https:\/\/www.uni-marburg.de\/en\/fb16\/ipc\/kolb-group\" target=\"_blank\" rel=\"noopener\">Homepage<\/a><\/p>\n<\/p><\/div><hr><\/div>\t\t\t <div class=\"project-content two_three border-left\"><p><strong>Project description:<\/strong><\/p>\n<p>Cellular redox balance plays an essential role in pathogenic microorganisms. Enzymes of the NAD(P)H-dependent glutathione and thioredoxin system<sup>1,2<\/sup>, as well as the glucose 6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD), which significantly contribute to the NADPH and ribose 5-phosphate pool via the pentose phosphate pathway<sup>3<\/sup>, are centrally involved. G6PD of the malaria parasite <em>Plasmodium falciparum<\/em> and <em>P. vivax<\/em> (GluPho), present as a bifunctional enzyme, differs functionally and structurally from the human host enzyme<sup>4<\/sup> and is essential for malaria parasites<sup>5<\/sup>. Together with the Sanford-Burnham Institute\/UCSD, La Jolla, we have established a high-throughput compatible assay for <em>Pf<\/em>GluPho and have screened about 400,000 compounds (i.a. NIH MLSMR Collection)<sup>6<\/sup>. Following structure-activity relationship studies and lead optimization, we identified the highly selective <em>Pf<\/em>GluPho inhibitor SBI-750, which is active in the lower nanomolar range<sup>7<\/sup>. The concept could already be transferred to <em>Leishmania;<\/em> the 3D crystal structure of <em>Leishmania donovani<\/em> G6PDs and PGDs could be solved and based on this a first <em>in silico<\/em> screening of small molecules could be realized. In addition, a high-throughput compatible assay for the recombinant G6PDs and 6PGDs from <em>Leishmania<\/em> was established to screen for inhibitors at the Novartis FAST lab in Cambridge (USA).<\/p>\n<div id=\"attachment_513\" style=\"width: 310px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-513\" class=\"wp-image-513 size-medium\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/\/b03-grafik-01-300x228.png\" alt=\"\" width=\"300\" height=\"228\" srcset=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/b03-grafik-01-300x228.png 300w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/b03-grafik-01.png 530w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><p id=\"caption-attachment-513\" class=\"wp-caption-text\">Workflow-Diagramm zur Inhibitor Identifizierung gegen G6PDs\/PGDs<\/p><\/div>\n<div id=\"attachment_511\" style=\"width: 310px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-511\" class=\"wp-image-511 size-medium\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/\/b03-grafik-02-300x218.png\" alt=\"\" width=\"300\" height=\"218\" srcset=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/b03-grafik-02-300x218.png 300w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/b03-grafik-02.png 428w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><p id=\"caption-attachment-511\" class=\"wp-caption-text\">3D-Kristallstruktur LdG6PD \u00a9Isabell Berneburg<\/p><\/div>\n<p><strong>Scientific goal: <\/strong><\/p>\n<p>The aim of this project is to functionally and structurally characterize the enzymes G6PD and 6PGD from <em>Leishmania<\/em> and <em>Plasmodium<\/em> as targets for drug development, and to identify and further develop inhibitors against these enzymes (<em>in silico<\/em> and HTS). The concept will also be transferred to other pathogens within the DRUID consortium, such as <em>Schistosoma<\/em>.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>DRUID Collaboration partners<\/strong><strong>:<\/strong><\/p>\n<p>B1 Diederich\/Kolb; B4 Grevelding; D3 van Zandbergen; E1 Grevelding\/H\u00e4berlein; E3 Rahlfs\/Przyborski; E4 Spengler; E6 Schiffmann\/Laux<\/p>\n<hr \/>\n<p>References B3: <strong>1. <\/strong>Fritz-Wolf <em>et al.<\/em> <strong>(2011) <\/strong><strong><em>Nature Comm.<\/em><\/strong> 2:383*<strong> 2.<\/strong> Koncarevic <em>et al.<\/em> <strong>(2009)<\/strong> <strong><em>PNAS <\/em><\/strong>106: 13323-8* <strong>3. <\/strong>Bozdech and Ginsburg <strong>(2005)<\/strong> <strong><em>Malaria J<\/em><\/strong> 3:23 <strong>4.<\/strong> Jortzik <em>et al.<\/em> <strong>(2011)<\/strong> <strong><em>Biochem J Energy<\/em><\/strong> 436:641-50* <strong>5<\/strong><strong>.<\/strong> Allen <em>et al.<\/em> <strong>(2015)<\/strong> <strong><em>FEBS J<\/em><\/strong> 282:3808-23*<br \/>\n<strong>6.<\/strong> Preuss <em>et al.<\/em> <strong>(2012)<\/strong> <strong><em>J Med Chem<\/em><\/strong> 55:7262-72* <strong>7. <\/strong>Berneburg <em>et al.<\/em><strong> (2022)<\/strong> <strong>Antimicrob Agents Chemother <\/strong>(accepted)*<\/p>\n<p>*own puplications<\/p>\n<\/div>            <\/div>        <\/div>    <\/div><\/section><section class=\"av_toggle_section\" >    <div role=\"tablist\" class=\"single_toggle\" data-tags=\"{Alle}\">        <p data-fake-id=\"#toggle-id-1\" class=\"toggler av-inherit-border-color\" role=\"tab\" tabindex=\"0\" aria-controls=\"1toggle-id-container\"><span class=\"project_nr\"> B4<\/span><span class=\"project_title\">Schistosoma mansoni: Biological Targets and Inhibitor Development<\/span><br><span class=\"project_names\">Christoph Grevelding<\/span><span class=\"toggle_icon\">        <span class=\"vert_icon\"><\/span><span class=\"hor_icon\"><\/span><\/span><\/p>        <div id=\"toggle-id-1-container\" class=\"toggle_wrap\">            <div class=\"toggle_content invers-color invers-color  av-inherit-border-color flex-container\">              <div class=\"projekt-names one_three\"><div class=\"project-name\"><div class=\"flex-container\"><div class=\"half\"><span class=\"principal\">Principal Investigator:<\/span><\/div><div class=\"half\"><a href=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/christoph-grevelding.jpg\"><img decoding=\"async\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/christoph-grevelding.jpg\" \/><\/a><\/div><\/div><p class=\"person_name\"><strong>Prof. Dr. Christoph Grevelding<\/strong><\/p><p><p>BFS, Institut f\u00fcr Parasitologie<br \/>\nJustus-Liebig-Universit\u00e4t Gie\u00dfen<br \/>\nSchubertstra\u00dfe 81<br \/>\n35392 Gie\u00dfen<br \/>\nTel.: +49 (0)641-99 38466<br \/>\nFax: +49 (0)641-99 38469<br \/>\nE-Mail: <a class=\"mail\" href=\"mailto:Christoph.Grevelding@vetmed.uni-giessen.de\">Christoph.Grevelding(at)vetmed.uni-giessen(dot)de<\/a><\/p>\n<\/p><\/div><hr><\/div>\t\t\t <div class=\"project-content two_three border-left\"><p><strong>Project description:<\/strong><\/p>\n<p><strong>Background: <\/strong>In the human and animal parasite <em>Schistosoma mansoni<\/em>, the sexual maturation of the female depends on a continuous pairing contact with the male (Abb. 1). Pairing is a prerequisite for egg produc-tion and as such decisive for the pathogenesis of schistosomiasis, which is induced by the eggs. Our research showed that different classes of molecules are involved in organizing reproductive processes in schisto-somes.<sup>1<\/sup> Among others, experiments with kinase inhibitors and by kinase RNA interference demonstrated that kinases regulate cell division, sper-matogenesis, oogenesis, egg production and vitality of schistosomes. For instance, the Abl-tyrosine kinase inhibitor imatinib (cancer drug Glivec) negatively affected reproduction and caused the degradation of the gastrodermis of adult <em>S.\u00a0mansoni<\/em> <em>in vitro<\/em> with lethal consequences.<sup>2,3<\/sup><\/p>\n<p>Besides kinases, which we study in cooperation with the Falcone group<sup>4<\/sup>, we investigate further enzymes such as aldehyde dehydrogenases (ALDHs) and an aldehyde reductase\u00a0(AR). In schistosomes, as in other organisms, these molecules are putatively involved in regulating responses to molecular stress. Inhibiting these molecules might devitalize the parasite. First <em>in vitro <\/em>experiments with inhibitors against these molecules, like the ALDH inhibitor disulfiram, caused morphologic alterations, a decrease of pairing stability, reduced vitality and finally, the death of adult schistosomes within days <em>in vitro<\/em>. Helicases might also represent interesting targets as suggested by studies using the RNA helicase-specific inhibitor Silvestrol.<sup>5 <\/sup>In adult <em>S.\u00a0mansoni<\/em>, Silvestrol reduced the vitality of adult worms <em>in vitro<\/em> but also stem cell-proliferation in gonad cells (Abb.\u00a02). Together with the working groups of Prof. Schlitzer and Prof. Gr\u00fcnweller (Marburg), we will investigate these potential target molecules and develop synthetic inhibitors<sup>6,7<\/sup> to study their physiological und morphological effects, first <em>in vitro<\/em>. Further, top candidates will be used for <em>in vivo<\/em> tests in a rodent infection model.<\/p>\n<div id=\"attachment_519\" style=\"width: 310px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-519\" class=\"wp-image-519 size-medium\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/\/b04-grafik-01-300x274.jpg\" alt=\"\" width=\"300\" height=\"274\" srcset=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/b04-grafik-01-300x274.jpg 300w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/b04-grafik-01.jpg 360w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><p id=\"caption-attachment-519\" class=\"wp-caption-text\">Abb. 1. Bright-field microscopy of a S. mansoni couple. During the constant pairing contact, the female (arrow) resides within the ventral groove of the male. Pairing is the essential pre-requisite for the production of eggs (stars).<\/p><\/div>\n<div id=\"attachment_521\" style=\"width: 150px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-521\" class=\"wp-image-521 size-full\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/b04-grafik-02.png\" alt=\"\" width=\"140\" height=\"113\" \/><p id=\"caption-attachment-521\" class=\"wp-caption-text\">Abb. 2. EdU-staining of female S. mansoni before (left) and after Silvestrol treatment (right). The num-ber of proliferating stem cells (green) is significantly reduced (right).<\/p><\/div>\n<p><strong>Scientific goal: <\/strong><\/p>\n<p>We will clone and characterize two RNA helicases of <em>S.\u00a0mansoni<\/em> at the molecular and biochemical levels. In different cooperations within DRUID (see below), we will test the recombinant proteins in enzyme assays against Silvestrol and synthetic derivatives of this substance. Furthermore, we continue our analyses of the recombinantly expressed enzymes <em>Sm<\/em>ALDH312 and <em>Sm<\/em>AR, further potential target molecules, and perform enzyme tests with inhibitors. Among these are disulfiram derivatives, which exhibited anti-schistosomal effects <em>in vitro<\/em> as shown in previous experiments. In addition, we plan to crystallize these molecules for structure analyses. Aim is to develop substances with inhibitor activity against the selected target molecules with high specificity, bioavailability and reduced toxicity for the host.<sup>8<\/sup>\u00a0Finally, results of the <em>in\u00a0vitro<\/em> and <em>in\u00a0vivo <\/em>experiments provide a basis for using this knowledge also for other parasitic systems, in which effective compounds will be tested (platform projekt E1).<\/p>\n<p>&nbsp;<\/p>\n<p><strong>DRUID Collaboration partners<\/strong><strong>:<\/strong><\/p>\n<p>A2 AG Gr\u00fcnweller, B3 AG Rahlfs\/Kolb\/van Zandbergen, B5 AG Schlitzer, E1 Platformprojekt\/H\u00e4berlein, E3 AG Rahlfs\/Przyborski, E5 AG Czermak\/Salzig lab<\/p>\n<hr \/>\n<p>References B4: <sup>1<\/sup>Beckmann et al. (2010) <em>PLoS Pathog<\/em> 6:e1000769; <sup>2<\/sup>Beckmann et al. (2010) <em>Int J Parasitol<\/em> 40:521-6; <sup>3<\/sup>Beckmann et al. (2012) <em>Curr Pharm Des<\/em> 18:3579-94; <sup>4<\/sup>Moreira et al. (2022) <em>Molecules<\/em> (in press); <sup>5<\/sup>Taroncher-Oldenburg et al. (2021) <em>Microorganisms<\/em> 9(3):540; <sup>6<\/sup>Peter Ventura et al. (2021) <em>ChemMedChem<\/em> 14(21):1856-62; <sup>7<\/sup>Peter Ventura et al. (2012) <em>Arch Pharm (Weinheim)<\/em> 354(12): e2100259; <sup>8<\/sup>M\u00e4der et al. (2018) <em>ChemMedChem<\/em> 13(22):2374-89.<\/p>\n<\/div>            <\/div>        <\/div>    <\/div><\/section><section class=\"av_toggle_section\" >    <div role=\"tablist\" class=\"single_toggle\" data-tags=\"{Alle}\">        <p data-fake-id=\"#toggle-id-1\" class=\"toggler av-inherit-border-color\" role=\"tab\" tabindex=\"0\" aria-controls=\"1toggle-id-container\"><span class=\"project_nr\"> B5<\/span><span class=\"project_title\">Development of Dithiocarbamates as Anthelminthics and Inhibitors of RNA-helicase eIF4A as potential antiviral Agents<\/span><br><span class=\"project_names\">Martin Schlitzer<\/span><span class=\"toggle_icon\">        <span class=\"vert_icon\"><\/span><span class=\"hor_icon\"><\/span><\/span><\/p>        <div id=\"toggle-id-1-container\" class=\"toggle_wrap\">            <div class=\"toggle_content invers-color invers-color  av-inherit-border-color flex-container\">              <div class=\"projekt-names one_three\"><div class=\"project-name\"><div class=\"flex-container\"><div class=\"half\"><span class=\"principal\">Principal Investigator:<\/span><\/div><div class=\"half\"><\/div><\/div><p class=\"person_name\"><strong>Prof. Dr. Martin Schlitzer<\/strong><\/p><p><p>Institut f\u00fcr Pharmazeutische Chemie<br \/>\nFachbereich Pharmazie<br \/>\nPhilipps-Universit\u00e4t Marburg<br \/>\nMarbacher Weg 6<br \/>\n35037 Marburg<br \/>\nTel.: +49 (0)6421 28-25840<br \/>\nE-Mail: <a href=\"mailto:schlitzer@staff.uni-marburg.de\">schlitzer(at)staff.uni-marburg(dot)de<\/a><\/p>\n<\/p><\/div><hr><\/div>\t\t\t <div class=\"project-content two_three border-left\"><p><strong>Project description:<\/strong><\/p>\n<p>Helminthic infections represent a major problem in large parts of the world. In comparison to the burden the arsenal of anthelminthic drugs is rather limited. Using successive cycles of design, synthesis and testing the novel class of dithiocarbamates shall be optimized regarding activity towards different helminths, host toxicity and drug-likeness. Derivatives are synthesized, tested against different helminths and human cell lines. For promising derivatives ADME-parameters are determined and selected compounds could be tested in vivo.<\/p>\n<p>Different viruses pathogenic to humans, as for instance the Ebola- or the SARS-CoV-2 virus, are using host factors for intracellular replication. These host factors therefor represent a target for anti-viral drug design. One of these host factors is the RNA-helicase eIF4A. Based on the crystal structure of the eIF4A-RNA complex appropriate ligands are designed, evaluated by docking and in case of proper fit synthesized. Compounds are tested in regard to their effect on translation efficiency. Active derivatives are subsequently evaluated regarding their effect on virus replication in cell cultures.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-527 size-full\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/b05-grafik-01.png\" alt=\"\" width=\"1351\" height=\"345\" srcset=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/b05-grafik-01.png 1351w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/b05-grafik-01-300x77.png 300w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/b05-grafik-01-1030x263.png 1030w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/b05-grafik-01-768x196.png 768w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/b05-grafik-01-705x180.png 705w\" sizes=\"auto, (max-width: 1351px) 100vw, 1351px\" \/><\/p>\n<p><strong>Scientific goal: <\/strong><\/p>\n<p>Both projects should yield compounds which possess the quality of a lead structure or possibly a development candidate<\/p>\n<p>&nbsp;<\/p>\n<p><strong>DRUID Collaboration partners<\/strong><strong>:<\/strong><\/p>\n<p>A2, A3, A4, A7, B2, B7P, C6 NWG, D4, E1, E4, E6<\/p>\n<hr \/>\n<p>References B5: <span style=\"font-size: 13.3333px;\">&#8211;<\/span><\/p>\n<\/div>            <\/div>        <\/div>    <\/div><\/section><section class=\"av_toggle_section\" >    <div role=\"tablist\" class=\"single_toggle\" data-tags=\"{Alle}\">        <p data-fake-id=\"#toggle-id-1\" class=\"toggler av-inherit-border-color\" role=\"tab\" tabindex=\"0\" aria-controls=\"1toggle-id-container\"><span class=\"project_nr\"> B6 P<\/span><span class=\"project_title\">Identification of antiviral targets in lipid metabolism<\/span><br><span class=\"project_names\">Eva Herker<\/span><span class=\"toggle_icon\">        <span class=\"vert_icon\"><\/span><span class=\"hor_icon\"><\/span><\/span><\/p>        <div id=\"toggle-id-1-container\" class=\"toggle_wrap\">            <div class=\"toggle_content invers-color invers-color  av-inherit-border-color flex-container\">              <div class=\"projekt-names one_three\"><div class=\"project-name\"><div class=\"flex-container\"><div class=\"half\"><span class=\"principal\">Principal Investigator:<\/span><\/div><div class=\"half\"><a href=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/eva-herker.jpg\"><img decoding=\"async\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/eva-herker.jpg\" \/><\/a><\/div><\/div><p class=\"person_name\"><strong>Prof. Dr. Eva Herker<\/strong><\/p><p><p>Institut f\u00fcr Virologie<br \/>\nPhilipps-Universit\u00e4t Marburg<br \/>\nHans-Meerwein-Str. 2<br \/>\n35043 Marburg<br \/>\nTel.: +49 (0)6421-28-64525<br \/>\nE-Mail: <a class=\"mail\" href=\"mailto:eva.herker@uni-marburg.de\">eva.herker(at)uni-marburg(dot)de<\/a><\/p>\n<\/p><\/div><hr><\/div>\t\t\t <div class=\"project-content two_three border-left\"><p><strong>Project description:<\/strong><\/p>\n<p>The human pathogenic flaviviruses Dengue (DENV), Yellow Fever (YFV), Zika (ZIKV), West Nile (WNV) and tick-borne encephalitis virus (TBEV) cause acute infections with severe complications. Fundamental steps in flavivirus replication are closely linked to <strong>cellular lipids<\/strong>. These include, among other things, membrane reorganizations for the formation of replication vesicles or capsid envelopment. Flaviviruses alter the lipid composition of the host cell, and the activity of various <strong>lipid-metabolizing enzymes<\/strong> is essential for successful replication. For this reason, enzymes from different lipid metabolism pathways represent interesting targets for <strong>(pan-) antiflaviviral therapy<\/strong>. However, it has not yet been clarified in detail whether the above-mentioned flaviviruses are dependent on different or similar branches of lipid metabolism.<\/p>\n<div id=\"attachment_535\" style=\"width: 970px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-535\" class=\"wp-image-535 size-full\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/b06p-grafik-01.jpg\" alt=\"\" width=\"960\" height=\"288\" srcset=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/b06p-grafik-01.jpg 960w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/b06p-grafik-01-300x90.jpg 300w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/b06p-grafik-01-768x230.jpg 768w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/b06p-grafik-01-705x212.jpg 705w\" sizes=\"auto, (max-width: 960px) 100vw, 960px\" \/><p id=\"caption-attachment-535\" class=\"wp-caption-text\">Immunofluorescence microscopy of cells infected with different flaviviruses; red: viral E protein; green: lipid droplets.<\/p><\/div>\n<p><strong>Scientific goal: <\/strong><\/p>\n<p>The project aims to identify antiviral targets in the cellular lipid metabolism. The role of various key enzymes in <em>de novo <\/em>fatty acid and cholesterol biosynthesis, in phospholipid and neutral lipid metabolism, and of lipid remodeling enzymes in flavivirus replication is analyzed using an shRNA-based screen. In addition, various inhibitors are tested. The results are validated in different cell types and the molecular mechanisms are examined in detail<\/p>\n<p>&nbsp;<\/p>\n<p><strong>DRUID Collaboration partners<\/strong><strong>:<\/strong><\/p>\n<p>B1 Diederich\/Kolb, D1 B\u00f6ttcher-Friebertsh\u00e4user\/Steinmetzer, E7P Krijnse-Locker, E4 Spengler<\/p>\n<hr \/>\n<p>References B6P: Herker<em> et al.<\/em> (<strong>2010<\/strong>) <strong><em>Nat Med<\/em> <\/strong>16: 1295 <strong>2. <\/strong>Harris<em> et al.<\/em> (<strong>2011<\/strong>) <strong><em>J Biol Chem<\/em> <\/strong>286: 42615 <strong>3. <\/strong>Herker<em> et al.<\/em> (<strong>2012<\/strong>) <strong><em>J Biol Chem<\/em> <\/strong>287: 2280 <strong>4. <\/strong>Rosch<em> et al.<\/em> (<strong>2016<\/strong>) <strong><em>Cell Rep<\/em> <\/strong>16: 3219 <strong>5. <\/strong>Hofmann<em> et al.<\/em> (<strong>2018<\/strong>) <strong><em>Biochim Biophys Acta Mol Cell Biol Lipids<\/em> <\/strong>1863: 1041 <strong>6. <\/strong>Schobel<em> et al.<\/em> (<strong>2018<\/strong>) <strong><em>Sci Rep<\/em> <\/strong>8: 3893 <strong>7. <\/strong>Lassen<em> et al.<\/em> (<strong>2019<\/strong>) <strong><em>J Cell Sci<\/em> <\/strong>132: jcs.217042 <strong>8. <\/strong>Bley<em> et al.<\/em> (<strong>2020<\/strong>) <strong><em>Int J Mol Sci<\/em> <\/strong>21:\u00a0 <strong>9. <\/strong>Herker<em> et al.<\/em> (<strong>2021<\/strong>) <strong><em>Trends Cell Biol<\/em> <\/strong>31: 345 <strong>10. <\/strong>Nguyen-Dinh<em> et al.<\/em> (<strong>2021<\/strong>) <strong><em>Cells<\/em> <\/strong>10: 2407<\/p>\n<\/div>            <\/div>        <\/div>    <\/div><\/section><section class=\"av_toggle_section\" >    <div role=\"tablist\" class=\"single_toggle\" data-tags=\"{Alle}\">        <p data-fake-id=\"#toggle-id-1\" class=\"toggler av-inherit-border-color\" role=\"tab\" tabindex=\"0\" aria-controls=\"1toggle-id-container\"><span class=\"project_nr\"> B7 P<\/span><span class=\"project_title\">New ways towards the control of schistosomiasis and echinococcosis<\/span><br><span class=\"project_names\">Franco Falcone<\/span><span class=\"toggle_icon\">        <span class=\"vert_icon\"><\/span><span class=\"hor_icon\"><\/span><\/span><\/p>        <div id=\"toggle-id-1-container\" class=\"toggle_wrap\">            <div class=\"toggle_content invers-color invers-color  av-inherit-border-color flex-container\">              <div class=\"projekt-names one_three\"><div class=\"project-name\"><div class=\"flex-container\"><div class=\"half\"><span class=\"principal\">Principal Investigator:<\/span><\/div><div class=\"half\"><a href=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/franco-falcone.jpg\"><img decoding=\"async\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/franco-falcone.jpg\" \/><\/a><\/div><\/div><p class=\"person_name\"><strong>Prof. Dr. Franco Falcone<\/strong><\/p><p><p>Institut f\u00fcr Parasitologie<br \/>\nBFS - Biomedizinisches Forschungszentrum Seltersberg<br \/>\nJustus-Liebig-Universit\u00e4t Gie\u00dfen<br \/>\nSchubertstra\u00dfe 81<br \/>\n35392 Gie\u00dfen<br \/>\nTel.: +49 (0)641-99 38030<br \/>\nE-Mail: <a class=\"mail\" href=\"mailto:franco.falcone@vetmed.uni-giessen.de\">franco.falcone(at)vetmed.uni-giessen(dot)de<\/a><\/p>\n<\/p><\/div><hr><\/div>\t\t\t <div class=\"project-content two_three border-left\"><p><strong>Project description:<\/strong><\/p>\n<p>Echinococcosis and Schistosomiasis are two co-called Neglected Tropical Diseases, which have a severe impact on the health of affected individuals in endemic countries. Our group is developing new tools that can be used for a better control of these two diseases. For schistosomiasis, we are developing new drugs that target the <strong>schistosomal Mitogen Activated Protein Kinases <\/strong>[1], \u00a0in an effort to find new drugs, which are larvicidal as well as adulticidal. For Echinococcosis, we are developing a novel diagnostic platform based on <strong>detection of parasite-specific IgE using humanized IgE reporter cell lines <\/strong>[2-4], which can be used in a variety of formats. We are also working on extending this diagnostic platform to other parasitic infections (e.g. <em>Fasciola<\/em>, Cysticercosis, <em>Clonorchis<\/em>, <em>Opisthorchis<\/em>).<\/p>\n<div id=\"attachment_372\" style=\"width: 310px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-372\" class=\"wp-image-372 size-medium\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/projekt-b07p-bild01-1-300x169.png\" alt=\"\" width=\"300\" height=\"169\" srcset=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/projekt-b07p-bild01-1-300x169.png 300w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/projekt-b07p-bild01-1.png 570w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><p id=\"caption-attachment-372\" class=\"wp-caption-text\">Larvicidal and adulticidal activity of compound 38_8, chosen from a compound library using our bioinformatic approach.<\/p><\/div>\n<div id=\"attachment_370\" style=\"width: 310px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-370\" class=\"wp-image-370 size-medium\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/projekt-b07p-bild01-300x280.png\" alt=\"\" width=\"300\" height=\"280\" srcset=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/projekt-b07p-bild01-300x280.png 300w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/projekt-b07p-bild01.png 456w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><p id=\"caption-attachment-370\" class=\"wp-caption-text\">Structural prediction of S. mansoni JNK compared to the human orthologue.<\/p><\/div>\n<p><strong>Scientific goal: <\/strong><\/p>\n<p>The Schistosome project aims to identify lead kinase inhibitor molecules using a bioinformatics <em>in silico<\/em> analysis pipeline and further develop these into suitable drugs, while in the Echinococcus project we are pursuing a better understanding of the protective immune responses against echinococci and aim to incorporate this knowledge into the development of vaccines and greatly improved, innovative diagnostic technologies.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>DRUID Collaboration partners<\/strong><strong>:<\/strong><\/p>\n<p>A4 Heine lab, B4 Grevelding lab, C6 NWG H\u00e4berlein lab, E5 Czermak\/Salzig lab<\/p>\n<hr \/>\n<p>References B7P: <strong>[1] <\/strong>*Pereira-Moreira\u00a0<em>et al.<\/em>\u00a0<strong>(2020)\u00a0<em>ACS Omega\u00a0<\/em><\/strong>5:9064-9070 [<strong>2]<\/strong> Kalli M <em>et al.<\/em> (2020) <strong>Sci Rep.<\/strong> 10:18208 <strong>[3]<\/strong> Kalli M. <em>et al.<\/em> (2020) <strong>Methods Mol Biol<\/strong>.;2163:155-162. [4] Prakash PS <em>et al.<\/em><strong> (2021)\u00a0 Parasitol Res.<\/strong><\/p>\n<\/div>            <\/div>        <\/div>    <\/div><\/section><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"parent":140,"menu_order":10,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-153","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.5 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Project area B - LOEWE-Zentrum DRUID<\/title>\n<meta name=\"description\" content=\"Find here detailed information Project area B of LOEWE-DRUID\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.loewe-druid.de\/en\/research\/project-area-b\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Project area B - 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