{"id":151,"date":"2021-12-06T12:17:15","date_gmt":"2021-12-06T11:17:15","guid":{"rendered":"http:\/\/wordpress.p604513.webspaceconfig.de\/research\/projektbereich-d\/"},"modified":"2023-11-07T12:16:27","modified_gmt":"2023-11-07T11:16:27","slug":"project-area-d","status":"publish","type":"page","link":"https:\/\/www.loewe-druid.de\/en\/research\/project-area-d\/","title":{"rendered":"Project area D"},"content":{"rendered":"\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-av_one_fourth-003d50325157ae76c6cf2b217f70282b\">\n.flex_column.av-av_one_fourth-003d50325157ae76c6cf2b217f70282b{\nborder-radius:0px 0px 0px 0px;\npadding:0px 0px 0px 0px;\n}\n<\/style>\n<div  class='flex_column av-av_one_fourth-003d50325157ae76c6cf2b217f70282b av_one_fourth  avia-builder-el-0  el_before_av_three_fourth  avia-builder-el-first  rahmen-headline-projects first flex_column_div av-zero-column-padding  '     ><style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-l0kzrmwv-fef8e3de08ef7c5b90ff9cddfb8e5072\">\n#top .av-special-heading.av-l0kzrmwv-fef8e3de08ef7c5b90ff9cddfb8e5072{\npadding-bottom:10px;\n}\nbody .av-special-heading.av-l0kzrmwv-fef8e3de08ef7c5b90ff9cddfb8e5072 .av-special-heading-tag .heading-char{\nfont-size:25px;\n}\n.av-special-heading.av-l0kzrmwv-fef8e3de08ef7c5b90ff9cddfb8e5072 .av-subheading{\nfont-size:15px;\n}\n<\/style>\n<div  class='av-special-heading av-l0kzrmwv-fef8e3de08ef7c5b90ff9cddfb8e5072 av-special-heading-h1 blockquote modern-quote  avia-builder-el-1  avia-builder-el-no-sibling '><h1 class='av-special-heading-tag '  itemprop=\"headline\"  >Project area D:<\/h1><div class=\"special-heading-border\"><div class=\"special-heading-inner-border\"><\/div><\/div><\/div><\/div>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-av_three_fourth-74b9a86a12f903134a10607d86902188\">\n.flex_column.av-av_three_fourth-74b9a86a12f903134a10607d86902188{\nborder-radius:0px 0px 0px 0px;\npadding:0px 0px 0px 0px;\n}\n<\/style>\n<div  class='flex_column av-av_three_fourth-74b9a86a12f903134a10607d86902188 av_three_fourth  avia-builder-el-2  el_after_av_one_fourth  avia-builder-el-last  rahmen-accordion-projects border-left flex_column_div av-zero-column-padding  '     ><div class=\"togglecontainer toggle_close_all  avia-builder-el-5  avia-builder-el-no-sibling  enable_toggles projekte_toggle\"><section class=\"av_toggle_section\" >    <div role=\"tablist\" class=\"single_toggle\" data-tags=\"{Alle}\">        <p data-fake-id=\"#toggle-id-1\" class=\"toggler av-inherit-border-color\" role=\"tab\" tabindex=\"0\" aria-controls=\"1toggle-id-container\"><span class=\"project_nr\"> D1<\/span><span class=\"project_title\">Inhibition of virus-activating host proteases<\/span><br><span class=\"project_names\">Eva Friebertsh\u00e4user, Torsten Steinmetzer<\/span><span class=\"toggle_icon\">        <span class=\"vert_icon\"><\/span><span class=\"hor_icon\"><\/span><\/span><\/p>        <div id=\"toggle-id-1-container\" class=\"toggle_wrap\">            <div class=\"toggle_content invers-color invers-color  av-inherit-border-color flex-container\">              <div class=\"projekt-names one_three\"><div class=\"project-name\"><div class=\"flex-container\"><div class=\"half\"><span class=\"principal\">Principal Investigator:<\/span><\/div><div class=\"half\"><a href=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/eva-boettcher-friebertshaeuser.jpg\"><img decoding=\"async\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/eva-boettcher-friebertshaeuser.jpg\" \/><\/a><\/div><\/div><p class=\"person_name\"><strong>Prof. Dr. Eva Friebertsh\u00e4user<\/strong><\/p><p><p>Institut f\u00fcr Virologie<br \/>\nPhilipps-Universit\u00e4t Marburg<br \/>\nHans-Meerwein-Str. 2<br \/>\n35043 Marburg<br \/>\nTel.: +49 (0)6421-28 66019<br \/>\nFax: +49 (0)6421-28 68962<br \/>\nE-Mail: <a class=\"mail\" title=\"\" href=\"mailto:friebertshaeuser@staff.uni-marburg.de\">friebertshaeuser(at)staff.uni-marburg(dot)de<\/a><\/p>\n<\/p><\/div><hr><div class=\"project-name\"><div class=\"flex-container\"><div class=\"half\"><span class=\"principal\">Principal Investigator:<\/span><\/div><div class=\"half\"><a href=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/torsten-steinmetzer.jpg\"><img decoding=\"async\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/torsten-steinmetzer.jpg\" \/><\/a><\/div><\/div><p class=\"person_name\"><strong>Prof. Dr. Torsten Steinmetzer<\/strong><\/p><p><p>Institut f\u00fcr Pharmazeutische Chemie<br \/>\nPhilipps-Universit\u00e4t Marburg<br \/>\nMarbacher Weg 10<br \/>\n35032 Marburg<br \/>\nTel.: +49 (0)6421-28 25900<br \/>\nFax: +49 (0)6421-28 25901<br \/>\nE-Mail: <a class=\"mail\" href=\"mailto:torsten.steinmetzer@staff.uni-marburg.de\">torsten.steinmetzer(at)staff.uni-marburg(dot)de<\/a><\/p>\n<\/p><\/div><hr><\/div>\t\t\t <div class=\"project-content two_three border-left\"><p><strong>Project description:<\/strong><\/p>\n<p>Cleavage of viral envelope proteins by host proteases is essential for the infectivity of many human pathogenic viruses. Among others, the surface glycoproteins of highly pathogenic avian influenza viruses (e.g. H5N1), chikungunya virus or dengue, West Nile and Zika viruses are activated by furin-like serine proteases. The surface glycoproteins hemagglutinin of zoonotic H7N9 and seasonal influenza A viruses or the spike protein S of many coronaviruses (CoV) are cleaved by the membrane-bound trypsin-like serine protease TMPRSS2. Recently, we were able to show that SARS-CoV-2 S is activated by both furin and TMPRSS2. Therefore, these host proteases are promising targets for the development of novel broad-spectrum antiviral agents.<\/p>\n<div id=\"attachment_556\" style=\"width: 426px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-556\" class=\"wp-image-556 size-full\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/d01-grafik-01.png\" alt=\"\" width=\"416\" height=\"277\" srcset=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/d01-grafik-01.png 416w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/d01-grafik-01-300x200.png 300w\" sizes=\"auto, (max-width: 416px) 100vw, 416px\" \/><p id=\"caption-attachment-556\" class=\"wp-caption-text\">Crystal structure of furin in complex with inhibitor MI-1851<\/p><\/div>\n<div id=\"attachment_558\" style=\"width: 426px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-558\" class=\"wp-image-558 size-full\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/d01-grafik-02.png\" alt=\"\" width=\"416\" height=\"277\" srcset=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/d01-grafik-02.png 416w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/d01-grafik-02-300x200.png 300w\" sizes=\"auto, (max-width: 416px) 100vw, 416px\" \/><p id=\"caption-attachment-558\" class=\"wp-caption-text\">Crystal structure of TMPRSS2 superimposed with inhibitors MI-1904 (yellow) and MI-432 (orange).<\/p><\/div>\n<p>&nbsp;<\/p>\n<p><strong>Scientific goal: <\/strong><\/p>\n<p>Structure-based development of effective inhibitors targeting virus-activating host proteases; determination of their potency and selectivity by enzyme kinetic studies; structural characterization of their binding mode in complex with relevant host proteases; testing of their antiviral efficacy against significant human pathogenic viruses in cell cultures, tissue cultures and animal models.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>DRUID Collaboration partners<\/strong><strong>:<\/strong><\/p>\n<p>A1 Becker lab, A2 Gr\u00fcnweller lab, B6 Herker lab, C1 Hildt lab<\/p>\n<hr \/>\n<p>References D1: <strong>1. <\/strong>B\u00f6ttcher <em>et al.<\/em><strong> (2006) <em>J Virol<\/em> <\/strong>80: 9896-8<strong> 3. <\/strong>Becker <em>et al.<\/em><strong> (2012) <em>J Biol Chem<\/em> <\/strong>287: 21992-03<strong> 4. <\/strong>B\u00f6ttcher-Friebertsh\u00e4user <em>et al.<\/em> <strong>(2012) <em>Vaccine<\/em> <\/strong>30: 7374-80<strong> 5. <\/strong>Ivanova <em>et al.<\/em><strong> (2017) <em>ChemMedChem<\/em> <\/strong>12: 1953-68<strong> 6. <\/strong>Lam van <em>et al. <strong>(2019) ChemMedChem <\/strong>14, 673-85<strong> 7. <\/strong>Bestle et al.<strong> (2020) LSA <\/strong>3: e202000786<strong>\u00a0 8. <\/strong>Bestle et al.<strong> (2021) J Virol<\/strong> 95: e0090621<strong> 9. <\/strong>Lam van et al.<strong> (2021) ACS Med Chem Lett <\/strong>12: 426-32.<\/em><\/p>\n<\/div>            <\/div>        <\/div>    <\/div><\/section><section class=\"av_toggle_section\" >    <div role=\"tablist\" class=\"single_toggle\" data-tags=\"{Alle}\">        <p data-fake-id=\"#toggle-id-1\" class=\"toggler av-inherit-border-color\" role=\"tab\" tabindex=\"0\" aria-controls=\"1toggle-id-container\"><span class=\"project_nr\"> D2<\/span><span class=\"project_title\">HEV, Identification of cellular targets for antiviral strategies-inhibition of virus release by modulation of cholesterol level<\/span><br><span class=\"project_names\">Kai-Henrik Peiffer, Stefan Zeuzem, Eberhard Hildt<\/span><span class=\"toggle_icon\">        <span class=\"vert_icon\"><\/span><span class=\"hor_icon\"><\/span><\/span><\/p>        <div id=\"toggle-id-1-container\" class=\"toggle_wrap\">            <div class=\"toggle_content invers-color invers-color  av-inherit-border-color flex-container\">              <div class=\"projekt-names one_three\"><div class=\"project-name\"><div class=\"flex-container\"><div class=\"half\"><span class=\"principal\">Principal Investigator:<\/span><\/div><div class=\"half\"><a href=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/kai-henrik-peiffer.jpg\"><img decoding=\"async\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/kai-henrik-peiffer.jpg\" \/><\/a><\/div><\/div><p class=\"person_name\"><strong>Dr. Kai-Henrik Peiffer<\/strong><\/p><p><p>Goethe-Universit\u00e4t Frankfurt am Main<br \/>\nUniversit\u00e4tsklinikum<br \/>\nZentrum der Inneren Medizin<br \/>\nMedizinische Klinik I<br \/>\nTheodor-Stern-Kai 7<br \/>\n60596 Frankfurt am Main<br \/>\nE-Mail: <a class=\"mail\" href=\"mailto:kai-henrik.peiffer@kgu.de\">kai-henrik.peiffer(at)kgu(dot)de<\/a><\/p>\n<\/p><\/div><hr><div class=\"project-name\"><div class=\"flex-container\"><div class=\"half\"><span class=\"principal\">Principal Investigator:<\/span><\/div><div class=\"half\"><a href=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/eberhard-hildt.jpg\"><img decoding=\"async\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/eberhard-hildt.jpg\" \/><\/a><\/div><\/div><p class=\"person_name\"><strong>Prof. Dr. Eberhard Hildt<\/strong><\/p><p><p>Bundesinstitut f\u00fcr Impfstoffe<br \/>\nund biomedizinische Arzneimittel<br \/>\nPaul-Ehrlich-Institut<br \/>\nPaul-Ehrlich-Stra\u00dfe 51-59<br \/>\n63225 Langen<br \/>\nTel.: +49 (0)6103-77 2140<br \/>\nFax: +49 (0)6103-77 1234<br \/>\nE-Mail: <a class=\"mail\" href=\"mailto:Eberhard.Hildt@pei.de\">Eberhard.Hildt(at)pei(dot)de<\/a><\/p>\n<p><a href=\"http:\/\/www.pei.de\/DE\/forschung\/forschungsgruppen\/virologie\/virologie-node\" target=\"_blank\" rel=\"noopener\">Homepage<\/a><\/p>\n<\/p><\/div><hr><div class=\"project-name\"><div class=\"flex-container\"><div class=\"half\"><span class=\"principal\">Principal Investigator:<\/span><\/div><div class=\"half\"><a href=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/stefan-zeuzem.jpg\"><img decoding=\"async\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/stefan-zeuzem.jpg\" \/><\/a><\/div><\/div><p class=\"person_name\"><strong>Prof. Dr. Stefan Zeuzem<\/strong><\/p><p><p>Department of Medicine<br \/>\nGoethe University Hospital<br \/>\nTheodor-Stern-Kai 7<br \/>\n60590 Frankfurt am Main<br \/>\nTel.: +49 (0)69-6301 4544<br \/>\nE-Mail: <a class=\"mail\" href=\"mailto:zeuzem@em.uni-frankfurt.de\">zeuzem(at)em.uni-frankfurt(dot)de<\/a><\/p>\n<\/p><\/div><hr><\/div>\t\t\t <div class=\"project-content two_three border-left\"><p><strong>Project description:<\/strong><\/p>\n<p>HEV infects more than 20 Mio people per year\u00a0 and concerns industrial nations as well as developing countries. At present there is no specific therapy available.As non-enveloped virus HEV release depends on endodomal processes which represent a promising target for antivirals. In the first funding period the lysosmal degradation of HEV in the endosomal system was identified as a central anchor point for antivirals. In addition to innate immunity cholesterol homeostasis is a a central factor affecting HEV reelase. Based on this the underlying mechanisms\u00a0 are analysed in detalil to identifiy further targets\u00a0 which can be addressed by drug repurposing. Animal models will help to broaden the insight in immunological processes controlling HEV life cycle.<\/p>\n<div id=\"attachment_571\" style=\"width: 756px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-571\" class=\"wp-image-571 size-full\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/d02-grafik-01.png\" alt=\"\" width=\"746\" height=\"424\" srcset=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/d02-grafik-01.png 746w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/d02-grafik-01-300x171.png 300w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/d02-grafik-01-705x401.png 705w\" sizes=\"auto, (max-width: 746px) 100vw, 746px\" \/><p id=\"caption-attachment-571\" class=\"wp-caption-text\">3D reconstruction of subcellular distribution of HEV (green) , GBP-1 (cyan) and lysosomes (red) in interferon \uf067-treated cells.<\/p><\/div>\n<div id=\"attachment_569\" style=\"width: 785px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-569\" class=\"wp-image-569 size-full\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/d02-grafik-02.png\" alt=\"\" width=\"775\" height=\"331\" srcset=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/d02-grafik-02.png 775w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/d02-grafik-02-300x128.png 300w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/d02-grafik-02-768x328.png 768w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/d02-grafik-02-705x301.png 705w\" sizes=\"auto, (max-width: 775px) 100vw, 775px\" \/><p id=\"caption-attachment-569\" class=\"wp-caption-text\">CLSM-based 3D reconstruction of sub-cellular distribution of HEV (green) and lysosomes (cyan) after induction of and cholesterol accumulation (magenta).<\/p><\/div>\n<p>&nbsp;<\/p>\n<p><strong>Scientific goal: <\/strong><\/p>\n<p>Inhibtion of the endosomal life cycle of HEV by modulation of cholesterol-dependent regulated target structurs via application of identified bioactive compounds (drug repurposing).<\/p>\n<p>&nbsp;<\/p>\n<p><strong>DRUID Collaboration partners<\/strong><strong>:<\/strong><\/p>\n<p>A2 Gr\u00fcnweller lab, A4 Heine\/Reuter lab, B1 Diederich\/Kolb lab, B6P Herker lab, C5 Glebe\/Geyer lab, D1 Steinmetzer lab<\/p>\n<hr \/>\n<p>References D2: <strong>\u00a0[1] <\/strong>Glitscher et al. (2018) <strong>Viruses <\/strong>10(6):301; <strong>[2<\/strong>] M\u00fcller et al. (2020) <strong>Antiviral Res<\/strong> 174:104706;<strong> [3] <\/strong>Basic et al. (2019) <strong>Antiviral Res<\/strong> 172:104644; <strong>[4] <\/strong>Glitscher et al. (2021) <strong>J Virol<\/strong> doi: 10.1128\/JVI.01564-20; <strong>[5]<\/strong> Glitscher et al. (2021) <strong>Cell Mol Gastroenterol Hepatol<\/strong>, doi:10.1016 \/j.jcmgh.2021.02.002; <strong>[6] <\/strong>Himmelsbach et al. (2018) <strong>Emerg Microbes Infect<\/strong> 7(1):196. <strong>[7] <\/strong>Glitscher et al. (2021) <strong>Cell Microbiol<\/strong>. 16:e13379<\/p>\n<\/div>            <\/div>        <\/div>    <\/div><\/section><section class=\"av_toggle_section\" >    <div role=\"tablist\" class=\"single_toggle\" data-tags=\"{Alle}\">        <p data-fake-id=\"#toggle-id-1\" class=\"toggler av-inherit-border-color\" role=\"tab\" tabindex=\"0\" aria-controls=\"1toggle-id-container\"><span class=\"project_nr\"> D3<\/span><span class=\"project_title\">Characterisation of apoptotic parasites to identify new target molecules for the treatment of Leishmaniasis<\/span><br><span class=\"project_names\">Ger van Zandbergen<\/span><span class=\"toggle_icon\">        <span class=\"vert_icon\"><\/span><span class=\"hor_icon\"><\/span><\/span><\/p>        <div id=\"toggle-id-1-container\" class=\"toggle_wrap\">            <div class=\"toggle_content invers-color invers-color  av-inherit-border-color flex-container\">              <div class=\"projekt-names one_three\"><div class=\"project-name\"><div class=\"flex-container\"><div class=\"half\"><span class=\"principal\">Principal Investigator:<\/span><\/div><div class=\"half\"><a href=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/ger-van-zandbergen.jpg\"><img decoding=\"async\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/ger-van-zandbergen.jpg\" \/><\/a><\/div><\/div><p class=\"person_name\"><strong>Prof. Dr. Ger van Zandbergen<\/strong><\/p><p><p>Abteilung Immunologie<br \/>\nPaul-Ehrlich-Institut<br \/>\nPaul-Ehrlich-Str. 51-59<br \/>\n63225 Langen<br \/>\nTel.: +49 (0)6103-77 2005<br \/>\nE-Mail: <a class=\"mail\" href=\"mailto:Ger.Zandbergen@pei.de\">Ger.Zandbergen(at)pei(dot)de<\/a><\/p>\n<\/p><\/div><hr><\/div>\t\t\t <div class=\"project-content two_three border-left\"><p><strong>Project description:<\/strong><\/p>\n<p>Leishmaniasis is a neglected tropical disease caused by the parasite <em>Leishmania spp<\/em>, which is endemic in almost 100 countries. We could demonstrate that apoptotic <em>Leishmania major (L. major)<\/em> promastigotes are both responsible for the infectivity and the survival of parasites in host cells. Apoptotic parasites induce an anti-inflammatory response in human macrophages that does not lead to an effective T-cell response against Leishmania. Even if <em>L. major<\/em> parasites show all typical characteristics of apoptosis, typical eukaryotic apoptosis regulating proteins are not present in Leishmania.<\/p>\n<div id=\"attachment_577\" style=\"width: 418px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-577\" class=\"wp-image-577 size-full\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/d03-grafik-01.png\" alt=\"\" width=\"408\" height=\"347\" srcset=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/d03-grafik-01.png 408w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/d03-grafik-01-300x255.png 300w\" sizes=\"auto, (max-width: 408px) 100vw, 408px\" \/><p id=\"caption-attachment-577\" class=\"wp-caption-text\">Staining of fragmented DNA (TUNEL staining) in L. major Cas9\/T7 in which apoptosis was induced by Miltefosin (TUNEL: green; DAPI: blue; anti-Lm-serum: red. \u00a9 Ger van Zandbergen)<\/p><\/div>\n<p>&nbsp;<\/p>\n<p><strong>Scientific goal: <\/strong><\/p>\n<p>For a novel vaccination approach and to kill parasites we aim to identify apoptosis regulating proteins in Leishmania as potential drug targets as well as attenuated Leishmania strains without anti-inflammatory properties.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>DRUID Collaboration partners<\/strong><strong>:<\/strong><\/p>\n<p>B1 Kolb, B3 Rahlfs\/Kolb, E3 Rahlfs\/Przyborski<\/p>\n<hr \/>\n<p>References D3: [1] Arens et al. <strong>(2018) <em>Front Immunol<\/em><\/strong> 31(9):1772. [2] Crauwels et al. <strong>(2019) <em>Front Immunol<\/em><\/strong> 22(10):2697. Further publications within DRUID: [3] Turo\u0148ov\u00e1 et al. <strong>(2020) <em>Science<\/em><\/strong> 370(6513):203-208.<\/p>\n<\/div>            <\/div>        <\/div>    <\/div><\/section><section class=\"av_toggle_section\" >    <div role=\"tablist\" class=\"single_toggle\" data-tags=\"{Alle}\">        <p data-fake-id=\"#toggle-id-1\" class=\"toggler av-inherit-border-color\" role=\"tab\" tabindex=\"0\" aria-controls=\"1toggle-id-container\"><span class=\"project_nr\"> D4<\/span><span class=\"project_title\">Blockage of glutaminolysis and glycolysis for Cryptosporidium parvum inhibition and One Health study on cryptosporidiosis in Cameroon<\/span><br><span class=\"project_names\">Carlos Hermosilla, Anja Taubert, Sybille Mazurek<\/span><span class=\"toggle_icon\">        <span class=\"vert_icon\"><\/span><span class=\"hor_icon\"><\/span><\/span><\/p>        <div id=\"toggle-id-1-container\" class=\"toggle_wrap\">            <div class=\"toggle_content invers-color invers-color  av-inherit-border-color flex-container\">              <div class=\"projekt-names one_three\"><div class=\"project-name\"><div class=\"flex-container\"><div class=\"half\"><span class=\"principal\">Principal Investigator:<\/span><\/div><div class=\"half\"><a href=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/sybille-mazurek.jpg\"><img decoding=\"async\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/sybille-mazurek.jpg\" \/><\/a><\/div><\/div><p class=\"person_name\"><strong>Prof. Dr. Sybille Mazurek<\/strong><\/p><p><p>Institut f\u00fcr Veterin\u00e4r-Physiologie und -Biochemie<br \/>\nFachbereich Veterin\u00e4rmedizin<br \/>\nJustus-Liebig-Universit\u00e4t Gie\u00dfen<br \/>\nFrankfurter Str. 100<br \/>\n35392 Gie\u00dfen<br \/>\nTel.: + 49 (0)641-99 38182<br \/>\nE-Mail <a class=\"mail\" href=\"mailto:Sybille.Mazurek@vetmed.uni-giessen.de\">Sybille.Mazurek(at)vetmed.uni-giessen(dot)de<\/a><\/p>\n<p><a href=\"https:\/\/www.uni-giessen.de\/fbz\/fb10\/institute_klinikum\/institute\/\" target=\"_blank\" rel=\"noopener\">Website<\/a><\/p>\n<\/p><\/div><hr><div class=\"project-name\"><div class=\"flex-container\"><div class=\"half\"><span class=\"principal\">Principal Investigator:<\/span><\/div><div class=\"half\"><\/div><\/div><p class=\"person_name\"><strong>Prof. Dr. Carlos Hermosilla<\/strong><\/p><p><p>Institut f\u00fcr Parasitologie\/Institut f\u00fcr Veterin\u00e4r-Physiologie und -Biochemie<br \/>\nJustus-Liebig-Universit\u00e4t Gie\u00dfen<br \/>\nSchubertstr. 81\/Frankfurter Str. 100<br \/>\nTel.: +49 (o)641-99 38461\/99 38182<br \/>\nE-Mail: <a href=\"mailto:Carlos.R.Hermosilla@vetmed.uni-giessen.de\">Carlos.R.Hermosilla(at)vetmed.uni-giessen(dot)de<\/a><\/p>\n<p><a href=\"https:\/\/www.uni-giessen.de\/fbz\/fb10\/institute_klinikum\/institute\/\" target=\"_blank\" rel=\"noopener\">Homepage<\/a><\/p>\n<\/p><\/div><hr><div class=\"project-name\"><div class=\"flex-container\"><div class=\"half\"><span class=\"principal\">Principal Investigator:<\/span><\/div><div class=\"half\"><a href=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/anja-taubert.jpg\"><img decoding=\"async\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/anja-taubert.jpg\" \/><\/a><\/div><\/div><p class=\"person_name\"><strong>Prof. Dr. Anja Taubert<\/strong><\/p><p><p>BFS, Institut f\u00fcr Parasitologie<br \/>\nJustus-Liebig-Universit\u00e4t Gie\u00dfen<br \/>\nSchubertstra\u00dfe 81<br \/>\n35392 Gie\u00dfen<br \/>\nTel.: +49 (0)641-99 38460<br \/>\nFax: +49 (0)641-99 38469<br \/>\nE-Mail: <a class=\"mail\" href=\"mailto:Anja.Taubert@vetmed.uni-giessen.de\">Anja.Taubert(at)vetmed.uni-giessen(dot)de<\/a><\/p>\n<p><a href=\"https:\/\/www.uni-giessen.de\/fbz\/fb10\/institute_klinikum\/institute\/\" target=\"_blank\" rel=\"noopener\">Website<\/a><\/p>\n<\/p><\/div><hr><\/div>\t\t\t <div class=\"project-content two_three border-left\"><p><strong>Project description:<\/strong><\/p>\n<p><em>Cryptosporidium<\/em> spp. are intestinal parasites which cause severe diarrhoea in humans, especially in HIV patients and young children. Especially in developing countries these protozoan infections induce high morbiditiy and mortality. So far, epidemiological factors contributing to cryptosporidiosis in underdeveloped countries \u2013 such as Cameroon &#8211; have insufficiently been studied. Currently available therapeutics show insufficient efficacies in the above mentioned risk groups. <em>Cryptosporidium<\/em> spp. are obligate intracellular protozoa which own minimal metabolic capacities. Consequently, to sustain their intracellular proliferation, these parasites have to modulate the host cellular metabolism. By characterizing metabolic signatures of <em>C. parvum<\/em>-infected host cells, we recently identified host cell metabolic reactions and pathways that are essential for parasite proliferation.<\/p>\n<div id=\"attachment_585\" style=\"width: 649px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-585\" class=\"wp-image-585 size-full\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/d04-grafik-01.png\" alt=\"\" width=\"639\" height=\"639\" srcset=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/d04-grafik-01.png 639w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/d04-grafik-01-300x300.png 300w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/d04-grafik-01-80x80.png 80w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/d04-grafik-01-36x36.png 36w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/d04-grafik-01-180x180.png 180w\" sizes=\"auto, (max-width: 639px) 100vw, 639px\" \/><p id=\"caption-attachment-585\" class=\"wp-caption-text\">Cryptosporidium parvum- (yellow) infected host cells (nuclei: blue), tomographic microscopie \u00a9 Juan V\u00e9lez<\/p><\/div>\n<div id=\"attachment_583\" style=\"width: 276px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-583\" class=\"wp-image-583 size-full\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/d04-grafik-02.png\" alt=\"\" width=\"266\" height=\"362\" srcset=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/d04-grafik-02.png 266w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/d04-grafik-02-220x300.png 220w\" sizes=\"auto, (max-width: 266px) 100vw, 266px\" \/><p id=\"caption-attachment-583\" class=\"wp-caption-text\">Inhibition of Cryptosporidium parvum via exemplary metabolic blockers (V\u00e9lez et al. 2021c)<\/p><\/div>\n<p>&nbsp;<\/p>\n<p><strong>Scientific goal: <\/strong><\/p>\n<p>This projects targets specific metabolic pathways of host cells (mainly glycolysis and glutaminolysis) by using new metabolic inhibitors or combinatory treatments. Moreover, a One Health study on several epidemiological factors of cryptosporidiosis in Cameroon is conducted.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>DRUID Collaboration partners<\/strong><strong>:<\/strong><\/p>\n<p>E4 Spengler lab<\/p>\n<hr \/>\n<p>References D4: <strong>1.\u00a0<\/strong>*V\u00e9lez\u00a0<em>et al.<\/em>\u00a0<strong>(2021)\u00a0<\/strong><em><strong>Pathogens<\/strong><\/em><strong><em>\u00a011<\/em><\/strong>(1):49<strong>.<\/strong> <strong>\u00a02.\u00a0<\/strong>\u00a0* V\u00e9lez\u00a0<em>et al.<\/em>\u00a0<strong>(2021)\u00a0<\/strong><strong><em>Biology<\/em><\/strong><em> 10<\/em>(10):963<strong><em>\u00a0<\/em>3.<\/strong>\u00a0** V\u00e9lez\u00a0<em>et al.<\/em>\u00a0<strong>(2021)\u00a0<\/strong><strong><em>Biology<\/em><\/strong><em> 10<\/em>(1):60<\/p>\n<\/div>            <\/div>        <\/div>    <\/div><\/section><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"parent":140,"menu_order":12,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-151","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.4 - 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