{"id":150,"date":"2021-12-06T12:17:31","date_gmt":"2021-12-06T11:17:31","guid":{"rendered":"http:\/\/wordpress.p604513.webspaceconfig.de\/research\/projektbereich-e\/"},"modified":"2023-11-07T12:16:27","modified_gmt":"2023-11-07T11:16:27","slug":"project-area-e","status":"publish","type":"page","link":"https:\/\/www.loewe-druid.de\/en\/research\/project-area-e\/","title":{"rendered":"Project area E"},"content":{"rendered":"\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-av_one_fourth-0b64c06e27751950152fa827539bad4d\">\n.flex_column.av-av_one_fourth-0b64c06e27751950152fa827539bad4d{\nborder-radius:0px 0px 0px 0px;\npadding:0px 0px 0px 0px;\n}\n<\/style>\n<div  class='flex_column av-av_one_fourth-0b64c06e27751950152fa827539bad4d av_one_fourth  avia-builder-el-0  el_before_av_three_fourth  avia-builder-el-first  rahmen-headline-projects first flex_column_div av-zero-column-padding avia-link-column av-column-link avia-link-column-hover  '    data-link-column-url=\"https:\/\/www.loewe-druid.de\/forschung\/projektbereich-e\/\"   ><a class=\"av-screen-reader-only\" href=https:\/\/www.loewe-druid.de\/forschung\/projektbereich-e\/ >Link to: Projektbereich E<\/a><style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-kzfo537d-b7e7cbfc8ac707435c332c03f207c008\">\n#top .av-special-heading.av-kzfo537d-b7e7cbfc8ac707435c332c03f207c008{\npadding-bottom:10px;\n}\nbody .av-special-heading.av-kzfo537d-b7e7cbfc8ac707435c332c03f207c008 .av-special-heading-tag .heading-char{\nfont-size:25px;\n}\n.av-special-heading.av-kzfo537d-b7e7cbfc8ac707435c332c03f207c008 .av-subheading{\nfont-size:15px;\n}\n<\/style>\n<div  class='av-special-heading av-kzfo537d-b7e7cbfc8ac707435c332c03f207c008 av-special-heading-h1 blockquote modern-quote  avia-builder-el-1  avia-builder-el-no-sibling '><h1 class='av-special-heading-tag '  itemprop=\"headline\"  >Project area E:<\/h1><div class=\"special-heading-border\"><div class=\"special-heading-inner-border\"><\/div><\/div><\/div><\/div>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-av_three_fourth-74b9a86a12f903134a10607d86902188\">\n.flex_column.av-av_three_fourth-74b9a86a12f903134a10607d86902188{\nborder-radius:0px 0px 0px 0px;\npadding:0px 0px 0px 0px;\n}\n<\/style>\n<div  class='flex_column av-av_three_fourth-74b9a86a12f903134a10607d86902188 av_three_fourth  avia-builder-el-2  el_after_av_one_fourth  avia-builder-el-last  rahmen-accordion-projects border-left flex_column_div av-zero-column-padding  '     ><div class=\"togglecontainer toggle_close_all  avia-builder-el-5  avia-builder-el-no-sibling  enable_toggles projekte_toggle\"><section class=\"av_toggle_section\" >    <div role=\"tablist\" class=\"single_toggle\" data-tags=\"{Alle}\">        <p data-fake-id=\"#toggle-id-1\" class=\"toggler av-inherit-border-color\" role=\"tab\" tabindex=\"0\" aria-controls=\"1toggle-id-container\"><span class=\"project_nr\"> E1<\/span><span class=\"project_title\">Platform Drug Testing Against Helminths<\/span><br><span class=\"project_names\">Simone H\u00e4berlein, Christoph Grevelding<\/span><span class=\"toggle_icon\">        <span class=\"vert_icon\"><\/span><span class=\"hor_icon\"><\/span><\/span><\/p>        <div id=\"toggle-id-1-container\" class=\"toggle_wrap\">            <div class=\"toggle_content invers-color invers-color  av-inherit-border-color flex-container\">              <div class=\"projekt-names one_three\"><div class=\"project-name\"><div class=\"flex-container\"><div class=\"half\"><span class=\"principal\">Principal Investigator:<\/span><\/div><div class=\"half\"><a href=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/simone-haeberlein.jpg\"><img decoding=\"async\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/simone-haeberlein.jpg\" \/><\/a><\/div><\/div><p class=\"person_name\"><strong>PD Dr. Simone H\u00e4berlein<\/strong><\/p><p><p>Justus-Liebig-Universit\u00e4t Gie\u00dfen<br \/>\nBFS, Institut f\u00fcr Parasitologie<br \/>\nSchubertstra\u00dfe 81<br \/>\n35392 Gie\u00dfen<br \/>\nTel.: +49 (0)641-99 38476<br \/>\nFax: +49 (0)641-99 38469<br \/>\nE-Mail:<span class=\"link-mailto\"> <a class=\"email-link\" title=\"\" href=\"mailto:Simone.Haeberlein@vetmed.uni-giessen.de\" target=\"_self\" rel=\"\">Simone.Haeberlein(at)vetmed.uni-giessen(dot)de<\/a><\/span><\/p>\n<p><a href=\"https:\/\/www.uni-giessen.de\/fbz\/fb10\/institute_klinikum\/institute\/parasitologie\/forschung\/schisto\/mitarbeiter\/haeberlein\" target=\"_blank\" rel=\"noopener\">Homepage<\/a><\/p>\n<\/p><\/div><hr><div class=\"project-name\"><div class=\"flex-container\"><div class=\"half\"><span class=\"principal\">Principal Investigator:<\/span><\/div><div class=\"half\"><a href=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/christoph-grevelding.jpg\"><img decoding=\"async\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/christoph-grevelding.jpg\" \/><\/a><\/div><\/div><p class=\"person_name\"><strong>Prof. Dr. Christoph Grevelding<\/strong><\/p><p><p>BFS, Institut f\u00fcr Parasitologie<br \/>\nJustus-Liebig-Universit\u00e4t Gie\u00dfen<br \/>\nSchubertstra\u00dfe 81<br \/>\n35392 Gie\u00dfen<br \/>\nTel.: +49 (0)641-99 38466<br \/>\nFax: +49 (0)641-99 38469<br \/>\nE-Mail: <a class=\"mail\" href=\"mailto:Christoph.Grevelding@vetmed.uni-giessen.de\">Christoph.Grevelding(at)vetmed.uni-giessen(dot)de<\/a><\/p>\n<\/p><\/div><hr><\/div>\t\t\t <div class=\"project-content two_three border-left\"><p><strong>Project description:<\/strong><\/p>\n<p>Targets of antiparasitic compounds are often conserved, which opens the possibility to achieve therapy of different parasitic infections with the same active compound. In platform project E1, we focus on parasitic worms (helminths), for which the discovery of new active compounds is particularly important because of resistances or suboptimal therapeutic successes of existing drugs. To this end, we implement <em>in vitro<\/em> screenings of compounds originating from DRUID projects to test their efficacy against the blood fluke <em>Schistosoma mansoni<\/em> (causing schistosomiasis) and the liver fluke <em>Fasciola hepatica<\/em> (causing fascioliasis). The platform project cooperates with several national and international partners, including colleagues in endemic countries in Asia and Africa, who open us test possibilities against numerous other helminths species.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-597 size-full\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/e01-grafik-01.png\" alt=\"\" width=\"602\" height=\"421\" srcset=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/e01-grafik-01.png 602w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/e01-grafik-01-300x210.png 300w\" sizes=\"auto, (max-width: 602px) 100vw, 602px\" \/><\/p>\n<div id=\"attachment_593\" style=\"width: 995px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-593\" class=\"wp-image-593 size-full\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/e01-grafik-02.jpg\" alt=\"\" width=\"985\" height=\"749\" srcset=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/e01-grafik-02.jpg 985w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/e01-grafik-02-300x228.jpg 300w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/e01-grafik-02-768x584.jpg 768w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/e01-grafik-02-705x536.jpg 705w\" sizes=\"auto, (max-width: 985px) 100vw, 985px\" \/><p id=\"caption-attachment-593\" class=\"wp-caption-text\">Strategy for identifying active compounds with broad antiparasitic efficacy against globally important helminths species. \u00a9Simone H\u00e4berlein<\/p><\/div>\n<p>&nbsp;<\/p>\n<p>We also investigate the mechanism of action for selected test compounds by applying various <em>in vitro<\/em> culture-based and imaging methods.<\/p>\n<div id=\"attachment_595\" style=\"width: 953px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-595\" class=\"wp-image-595 size-full\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/e01-grafik-03.png\" alt=\"\" width=\"943\" height=\"308\" srcset=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/e01-grafik-03.png 943w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/e01-grafik-03-300x98.png 300w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/e01-grafik-03-768x251.png 768w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/e01-grafik-03-705x230.png 705w\" sizes=\"auto, (max-width: 943px) 100vw, 943px\" \/><p id=\"caption-attachment-595\" class=\"wp-caption-text\">Test spectrum provided by the platform project to identify and characterize anthelminthic compounds. \u00a9Simone H\u00e4berlein, Miray Tonk-R\u00fcgen<\/p><\/div>\n<p><strong>Scientific goal: <\/strong><\/p>\n<p>Aim of the project is the identification of substances with a broad anti-parasitic efficacy and the discovery of conserved modes of action.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>DRUID Collaboration partners<\/strong><strong>:<\/strong><\/p>\n<p>A2 Gr\u00fcnweller lab, A6 Douglas lab,\u00a0A7 Przyborski lab, B4 Schlitzer lab,\u00a0B5 Grevelding lab, B7 Falcone lab,\u00a0E4 Spengler lab<\/p>\n<hr \/>\n<p>References E1: <strong>1. <\/strong>Peter-Ventura <em>et al<\/em>. <strong>(2019) <em>ChemMedChem <\/em><\/strong>14(21):1856-1862. <strong>2.<\/strong> Houhou <em>et al.<\/em> <strong>(2019)<\/strong> <strong><em>Sci Rep<\/em><\/strong> 9:15867. <strong>3. <\/strong>Li <em>et al.<\/em> <strong>(2019) <em>Parasitol Res<\/em><\/strong> 118(3):881-890. <strong>4.<\/strong> Morawietz <em>et al.<\/em> <strong>(2020)<\/strong> <strong><em>Front Vet Sci<\/em><\/strong> 7:611270. <strong>5. <\/strong>Kellershohn <em>et al.<\/em> <strong>(2019)<\/strong> <strong><em>PLOS Negl Trop Dis<\/em><\/strong> 13:e0007240. <strong>6. <\/strong>Tonk <em>et al.<\/em> <strong>(2020)<\/strong> <strong><em>Antibiotics<\/em><\/strong> 9:664. <strong>7. <\/strong>Mokosch <em>et al.<\/em> <strong><em>Anal Bioanal Chem<\/em><\/strong> 413(10): 2755-2766. <strong>8.<\/strong> Mughal <em>et al.<\/em> <strong>(2021a) <em>Int J Parasitol<\/em><\/strong> 51(7):571-585. <strong>9. <\/strong>Mughal <em>et al.<\/em> <strong>(2021b) <em>Int J Parasitol<\/em><\/strong> S0020-7519(21)00312-X. <strong>10.<\/strong> Gallinger <em>et al.<\/em> <strong>(2022) <em>Pharmaceuticals<\/em><\/strong> 15(2): 119. <strong>11.<\/strong> Morawietz <em>et al.<\/em> <strong>(2022) <em>Parasitol Res<\/em><\/strong> (online ahead of print) doi: 10.1007\/s00436-021-07388-1<\/p>\n<\/div>            <\/div>        <\/div>    <\/div><\/section><section class=\"av_toggle_section\" >    <div role=\"tablist\" class=\"single_toggle\" data-tags=\"{Alle}\">        <p data-fake-id=\"#toggle-id-1\" class=\"toggler av-inherit-border-color\" role=\"tab\" tabindex=\"0\" aria-controls=\"1toggle-id-container\"><span class=\"project_nr\"> E3<\/span><span class=\"project_title\">Protein production, assay development, crystallisation and interaction analysis<\/span><br><span class=\"project_names\">Jude Przyborski, Stefan Rahlfs<\/span><span class=\"toggle_icon\">        <span class=\"vert_icon\"><\/span><span class=\"hor_icon\"><\/span><\/span><\/p>        <div id=\"toggle-id-1-container\" class=\"toggle_wrap\">            <div class=\"toggle_content invers-color invers-color  av-inherit-border-color flex-container\">              <div class=\"projekt-names one_three\"><div class=\"project-name\"><div class=\"flex-container\"><div class=\"half\"><span class=\"principal\">Principal Investigator:<\/span><\/div><div class=\"half\"><a href=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/jude-przyborski.jpg\"><img decoding=\"async\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/jude-przyborski.jpg\" \/><\/a><\/div><\/div><p class=\"person_name\"><strong>Prof. Dr. Jude Przyborski<\/strong><\/p><p><p>Interdisziplin\u00e4res Forschungszentrum (iFZ)<br \/>\nJustus-Liebig-Universit\u00e4t Gie\u00dfen<br \/>\nHeinrich-Buff-Ring 26-32<br \/>\n35392 Gie\u00dfen<br \/>\nTel.: +49 (0)641-99 39114<br \/>\nE-Mail: <a class=\"mail \" href=\"mailto:jude.przyborski@ernaehrung.uni-giessen.de\">jude.przyborski(at)ernaehrung.uni-giessen(dot)de<\/a><\/p>\n<p><a href=\"https:\/\/www.uni-giessen.de\/fbz\/fb09\/institute\/ernaehrungswissenschaft\/prof\/becker\/mitarbeiter\/jude\">Homepage<\/a><\/p>\n<\/p><\/div><hr><div class=\"project-name\"><div class=\"flex-container\"><div class=\"half\"><span class=\"principal\">Principal Investigator:<\/span><\/div><div class=\"half\"><a href=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/stefan-rahlfs.jpg\"><img decoding=\"async\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/stefan-rahlfs.jpg\" \/><\/a><\/div><\/div><p class=\"person_name\"><strong>Dr. Stefan Rahlfs<\/strong><\/p><p><p>Biochemie und Molekularbiologie<br \/>\nJustus-Liebig-Universit\u00e4t Gie\u00dfen<br \/>\nHeinrich-Buff-Ring 26-32<br \/>\n35390 Gie\u00dfen<br \/>\nTel.: +49 (0)641-99 39117<br \/>\nE-Mail: <a class=\"mail\" href=\"mailto:stefan.rahlfs@ernaehrung.uni-giessen.de\">stefan.rahlfs(at)ernaehrung.uni-giessen(dot)de<\/a><\/p>\n<\/p><\/div><hr><\/div>\t\t\t <div class=\"project-content two_three border-left\"><p><strong>Project description:<\/strong><\/p>\n<p>Within the framework of this platform project, we offer methods for protein production, for the development of high-throughput assays, for crystallisation and structural analysis as well as for interaction analyses. A variety of optimisation options for cloning\/expression and purification of native proteins (affinity chromatography, gel filtration, ion exchange) are available. Once recombinant protein has been obtained, provide support in the development of assay systems up to high throughput formats. Furthermore, the platform proves access to the crystallisation platform at the iFZ. A pipetting and a crystallisation robot as well as starter kits and various additive screens for optimisation are available for crystallisation screens on the nl scale. The system is currently being updated with funding from the latest DRUID funding round. Dr Fritz-Wolf acts as crystallographer and contact person and can carry out the first tests on the crystals. The X-ray sources of the Max Planck Institute for Medical Research (Heidelberg) and the AG Klebe\/Heine [A5] (Marburg) are available for this purpose.<\/p>\n<div id=\"attachment_397\" style=\"width: 183px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-397\" class=\"wp-image-397 size-full\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/projekt-e03-bild01.png\" alt=\"\" width=\"173\" height=\"209\" \/><p id=\"caption-attachment-397\" class=\"wp-caption-text\">Crystal structure of sfroGFP (Heimsch et al. 2022)<\/p><\/div>\n<p><strong>DRUID Collaboration partners<\/strong><strong>:<\/strong><\/p>\n<p>Protein-Expression: A1, A3, A6NWG, A7, B2, B3, B4, C3<br \/>\nCrystallisation: B2, B3, C3<br \/>\nAssay-Devlopment: A7, B3<\/p>\n<hr \/>\n<p>References E3:<strong> 1. <\/strong>Heimsch <em>et al.<\/em> <strong>(2022)<\/strong> <strong>Antioxid &amp; Redox Signal<\/strong> doi: 10.1089\/ars.2021.0234 <strong>2.<\/strong> Harnischfeger <em>et al.<\/em> <strong>(2020)<\/strong> <strong>Electronic Journal of Biotechnology<\/strong> 3<strong>.<\/strong> Fritz-Wolf <em>et al.<\/em> <strong>(2011) <\/strong><strong><em>Nat Commun<\/em><\/strong> 2:383<strong> 4.<\/strong> Koncarevic <em>et al.<\/em> <strong>(2009)<\/strong> <strong><em>Proc Natl Acad Sci USA<\/em><\/strong> 106:13323-8<\/p>\n<\/div>            <\/div>        <\/div>    <\/div><\/section><section class=\"av_toggle_section\" >    <div role=\"tablist\" class=\"single_toggle\" data-tags=\"{Alle}\">        <p data-fake-id=\"#toggle-id-1\" class=\"toggler av-inherit-border-color\" role=\"tab\" tabindex=\"0\" aria-controls=\"1toggle-id-container\"><span class=\"project_nr\"> E4<\/span><span class=\"project_title\">High resolution mass spectrometry imaging<\/span><br><span class=\"project_names\">Bernhard Spengler<\/span><span class=\"toggle_icon\">        <span class=\"vert_icon\"><\/span><span class=\"hor_icon\"><\/span><\/span><\/p>        <div id=\"toggle-id-1-container\" class=\"toggle_wrap\">            <div class=\"toggle_content invers-color invers-color  av-inherit-border-color flex-container\">              <div class=\"projekt-names one_three\"><div class=\"project-name\"><div class=\"flex-container\"><div class=\"half\"><span class=\"principal\">Principal Investigator:<\/span><\/div><div class=\"half\"><a href=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/bernhard-spengler-rkw-221110-05399-scaled.jpg\"><img decoding=\"async\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/bernhard-spengler-rkw-221110-05399-scaled.jpg\" \/><\/a><\/div><\/div><p class=\"person_name\"><strong>Prof. Dr. Bernhard Spengler<\/strong><\/p><p><p>Institut f\u00fcr Anorganische und Analytische Chemie<br \/>\nJustus-Liebig-Universit\u00e4t Gie\u00dfen<br \/>\nHeinrich-Buff-Ring 17<br \/>\n35392 Gie\u00dfen<br \/>\nTel.: +49 (0)641-99 34801<br \/>\nE-Mail: <a href=\"mailto:bernhard.spengler@ac.jlug.de\">bernhard.spengler(at)ac.jlug(dot)de<\/a><\/p>\n<p><a href=\"https:\/\/www.uni-giessen.de\/fbz\/fb08\/Inst\/iaac\/spengler\" target=\"_blank\" rel=\"noopener\">Homepage<\/a><\/p>\n<\/p><\/div><hr><\/div>\t\t\t <div class=\"project-content two_three border-left\"><p><strong>Project description:<\/strong><\/p>\n<p>High resolution mass spectrometry imaging provides numerous opportunities to aid in the characterization of pathogens, host-pathogen interactions and the analysis of drug molecules. The sample is analyzed with a laser in a rasterized fashion, the step size is only a few micrometers. The sample material is ablated and ionized, allowing for the parallel detection of hundreds of chemical compounds as well as the creation of images that show the distribution of the analytes throughout the sample.\u00a0 Identification of the molecules is carried out using exact mass determination and is supported by fragmentation experiments. Continued improvements in instrumentation allow for analysis and graphical representation of smaller structures while additional sample preparation steps like in-situ derivatization provide the opportunity to detect analytes at very low concentrations.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-603 size-full\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/e04-grafik-01.png\" alt=\"\" width=\"1361\" height=\"430\" srcset=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/e04-grafik-01.png 1361w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/e04-grafik-01-300x95.png 300w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/e04-grafik-01-1030x325.png 1030w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/e04-grafik-01-768x243.png 768w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/e04-grafik-01-705x223.png 705w\" sizes=\"auto, (max-width: 1361px) 100vw, 1361px\" \/><\/p>\n<p>&nbsp;<\/p>\n<p><strong>Scientific goal: <\/strong><\/p>\n<p>The goal of the project is the improvement and application of the available methods for the demands of the DRUID center. Especially the distribution of drug compounds, the lipidomic and metabolomic characterization of pathogens and the interaction of pathogens and hosts are important research areas.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>DRUID Collaboration partners<\/strong><strong>:<\/strong><\/p>\n<p>A2 Gr\u00fcnweller lab, B4 Grevelding lab, B5 Schlitzer lab,\u00a0D4 Taubert lab, E1 H\u00e4berlein lab, W3 Herker lab<\/p>\n<hr \/>\n<p>References E4: [1] Kadesch<em> et al. <\/em><strong><em>(2020<\/em><\/strong><strong>) PLoS Negl. Trop. Dis.<\/strong> 14(5): e0008145; [2] Morawietz<em> et al. <\/em>(<strong>2020)<\/strong>, <strong>Front. Vet. Sci.<\/strong> 7, 611270; [3] Mokosch<em> et al. <\/em><strong>(2021) \u00a0Anal Bioanal Chem<\/strong><em> <strong>413<\/strong><\/em><strong>, <\/strong>2755\u20132766; [4] M\u00fcller<em> et al. <\/em><strong>(<em>2021<\/em>) J. Am. Soc. Mass Spectrom.,3,32(2),465-472<\/strong>; [5] Spengler <em>et al.<\/em><strong> (2015<\/strong>) <strong>Anal Chem<\/strong> 87 (1) 64-82.<\/p>\n<\/div>            <\/div>        <\/div>    <\/div><\/section><section class=\"av_toggle_section\" >    <div role=\"tablist\" class=\"single_toggle\" data-tags=\"{Alle}\">        <p data-fake-id=\"#toggle-id-1\" class=\"toggler av-inherit-border-color\" role=\"tab\" tabindex=\"0\" aria-controls=\"1toggle-id-container\"><span class=\"project_nr\"> E5<\/span><span class=\"project_title\">Process development\/ Process control (PAT, cGMP), Production platform for proteins and virus-like particles<\/span><br><span class=\"project_names\">Peter Czermak, Denise Salzig<\/span><span class=\"toggle_icon\">        <span class=\"vert_icon\"><\/span><span class=\"hor_icon\"><\/span><\/span><\/p>        <div id=\"toggle-id-1-container\" class=\"toggle_wrap\">            <div class=\"toggle_content invers-color invers-color  av-inherit-border-color flex-container\">              <div class=\"projekt-names one_three\"><div class=\"project-name\"><div class=\"flex-container\"><div class=\"half\"><span class=\"principal\">Principal Investigator:<\/span><\/div><div class=\"half\"><a href=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/peter-czermak.jpg\"><img decoding=\"async\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/peter-czermak.jpg\" \/><\/a><\/div><\/div><p class=\"person_name\"><strong>Prof. Dr. Ing. Peter Czermak<\/strong><\/p><p><p>Institut f\u00fcr Bioprozesstechnik<br \/>\nTechnische Hochschule Mittelhessen<br \/>\nWiesenstra\u00dfe 14<br \/>\n35390 Gie\u00dfen<br \/>\nTel.: +49 (0)641-309 2551<br \/>\nE-Mail: <a class=\"mail\" href=\"mailto:peter.czermak@lse.thm.de\">peter.czermak(at)lse.thm(dot)de<\/a><\/p>\n<\/p><\/div><hr><div class=\"project-name\"><div class=\"flex-container\"><div class=\"half\"><span class=\"principal\">Principal Investigator:<\/span><\/div><div class=\"half\"><a href=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/denise-salzig.jpg\"><img decoding=\"async\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/denise-salzig.jpg\" \/><\/a><\/div><\/div><p class=\"person_name\"><strong>Prof. Dr. Ing. Denise Salzig<\/strong><\/p><p><p>Institut f\u00fcr Bioverfahrenstechnik<br \/>\nund Pharmazeutische Technologie<br \/>\nTechnische Hochschule Mittelhessen<br \/>\nGutfleischstr. 3-5<br \/>\nTel.: +49 (0)641-309 2630<br \/>\nFax: +49 (0)641-309 2553<br \/>\nE-Mail: <a class=\"mail\" href=\"mailto:denise.salzig@lse.thm.de\">denise.salzig(at)lse.thm(dot)de<\/a><\/p>\n<\/p><\/div><hr><\/div>\t\t\t <div class=\"project-content two_three border-left\"><p><strong>Project description:<\/strong><\/p>\n<p>For a translation of research results from the DRUID consortium into the clinic or industry, <strong>robust production processes, which<\/strong> represent more than just scaling up, are essential. These processes must follow the guidelines of good manufacturing practice (cGMP) and process analytical technology (PAT). The following work is planned: i) process intensification and expansion of the <strong>BEVS production platform<\/strong> as well as implementation of a continuous process control, ii) integration of online PAT technology, e.g., impedance spectroscopy for an automated determination of the optimal point in time for harvesting and automated harvesting, iii) expression (BEVS) and purification of <em>S. mansoni<\/em> kinases as well as study of the kinases and putative inhibitors, and iv) study of novel transfection reagents for <strong>transient protein production on a bioreactor scale<\/strong>.<\/p>\n<div id=\"attachment_613\" style=\"width: 937px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-613\" class=\"wp-image-613 size-full\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/e05-grafik-01.png\" alt=\"\" width=\"927\" height=\"409\" srcset=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/e05-grafik-01.png 927w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/e05-grafik-01-300x132.png 300w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/e05-grafik-01-768x339.png 768w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/e05-grafik-01-705x311.png 705w\" sizes=\"auto, (max-width: 927px) 100vw, 927px\" \/><p id=\"caption-attachment-613\" class=\"wp-caption-text\">Optimization of the transient transfection process using statistical design of experiments. \u00a9IBPT<\/p><\/div>\n<div id=\"attachment_611\" style=\"width: 373px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-611\" class=\"wp-image-611 size-full\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/e05-grafik-02.png\" alt=\"\" width=\"363\" height=\"426\" srcset=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/e05-grafik-02.png 363w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/e05-grafik-02-256x300.png 256w\" sizes=\"auto, (max-width: 363px) 100vw, 363px\" \/><p id=\"caption-attachment-611\" class=\"wp-caption-text\">Production concept with integrated PAT technology. \u00a9IBPT<\/p><\/div>\n<p>&nbsp;<\/p>\n<p><strong>Scientific goal: <\/strong><\/p>\n<p>The work on production process development and process control will be further adapted to the questions of the center, expanded, and offered to the entire consortium for use. It is planned to further intensify and automate the established BEVS (Baculovirus Expression System) platform. In addition, the second production platform \u2013 the transient production with HEK-293T cells &#8211; is also being further developed.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>DRUID Collaboration partners<\/strong><strong>:<\/strong><\/p>\n<p>B4 Schlitzer Lab, B5 Grevelding lab, B7 P Falcone Lab, C6 NWG H\u00e4berlein, E1 Platform Grevelding\/ H\u00e4berlein<\/p>\n<hr \/>\n<p>References E5: <strong>Biotechnol<\/strong>, DOI: 10.1016\/j.ejbt.2021.08.002, <strong>3.<\/strong> Lothert et al. <strong>(2020) Methods Mol Biol<\/strong> 2183:217-248, <strong>4.<\/strong> Dekevic et al. <strong>(2022)<\/strong> <strong>J Biotechn<\/strong> 346 23-34, <strong>5.<\/strong> Schwarz et al. <strong>(2021) Elec J Biotechnol<\/strong>, DOI: 10.1016\/j.ejbt.2022.01.003; <strong>6.<\/strong> Barekzai et al. (<strong>2020) New Adv Ferm Processes<\/strong>, DOI: 10.5772\/intechopen.90029, <strong>7. <\/strong>Eckhardt et al.<strong> (2021) Sep Sci Technol<\/strong>, 57 (6) 886-897<\/p>\n<\/div>            <\/div>        <\/div>    <\/div><\/section><section class=\"av_toggle_section\" >    <div role=\"tablist\" class=\"single_toggle\" data-tags=\"{Alle}\">        <p data-fake-id=\"#toggle-id-1\" class=\"toggler av-inherit-border-color\" role=\"tab\" tabindex=\"0\" aria-controls=\"1toggle-id-container\"><span class=\"project_nr\"> E6<\/span><span class=\"project_title\">Pharmaceutical development, translational medicine and drug repurposing<\/span><br><span class=\"project_names\">Susanne Schiffmann<\/span><span class=\"toggle_icon\">        <span class=\"vert_icon\"><\/span><span class=\"hor_icon\"><\/span><\/span><\/p>        <div id=\"toggle-id-1-container\" class=\"toggle_wrap\">            <div class=\"toggle_content invers-color invers-color  av-inherit-border-color flex-container\">              <div class=\"projekt-names one_three\"><div class=\"project-name\"><div class=\"flex-container\"><div class=\"half\"><span class=\"principal\">Principal Investigator:<\/span><\/div><div class=\"half\"><a href=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/dr-susanneschiffmann.png\"><img decoding=\"async\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/dr-susanneschiffmann.png\" \/><\/a><\/div><\/div><p class=\"person_name\"><strong>Dr. Susanne Schiffmann<\/strong><\/p><p><p>Fraunhofer Institut f\u00fcr Translationale Medizin und Pharmakologie ITMP<br \/>\nTheodor-Stern-Kai 7<br \/>\n60596 Frankfurt am Main<br \/>\nTel.: +49 (0)69-8700 25060<br \/>\nE-Mail: <a href=\"mailto:susanne.schiffmann@itmp.fraunhofer.de\">susanne.schiffmann(at)itmp.fraunhofer(dot)de<\/a><\/p>\n<p><a href=\"http:\/\/www.itmp.fraunhofer.de\/de\/innovationsbereiche\/krankheitsmodelle.html\" target=\"_blank\" rel=\"noopener\">Homepage<\/a><\/p>\n<\/p><\/div><hr><\/div>\t\t\t <div class=\"project-content two_three border-left\"><p><strong>Project description:<\/strong><\/p>\n<p>The SARS-CoV2 pandemic clearly demonstrates that there is an unmet medical need for anti-pathogenic drugs. In order to identify new treatment options, promising substances from other working groups will be characterized preclinical. This includes the generation of a safety profile and\/or bioavailability testing of the drug candidates. Since supportive therapy with immune-modulating drugs is a proven therapeutic approach, an interaction of the drug candidates with the immune system will be tested.<\/p>\n<div id=\"attachment_349\" style=\"width: 193px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-349\" class=\"wp-image-349 size-full\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/projekt-e06-bild01.jpg\" alt=\"\" width=\"183\" height=\"146\" \/><p id=\"caption-attachment-349\" class=\"wp-caption-text\">Surface marker expression of treated M2 macrophages \u00a9Marina Henke<\/p><\/div>\n<div id=\"attachment_347\" style=\"width: 310px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-347\" class=\"wp-image-347 size-medium\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/projekt-f06-bild02-300x241.png\" alt=\"\" width=\"300\" height=\"241\" srcset=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/projekt-f06-bild02-300x241.png 300w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/projekt-f06-bild02-1030x826.png 1030w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/projekt-f06-bild02-768x616.png 768w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/projekt-f06-bild02-1536x1232.png 1536w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/projekt-f06-bild02-1500x1203.png 1500w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/projekt-f06-bild02-495x400.png 495w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/projekt-f06-bild02-705x566.png 705w, https:\/\/www.loewe-druid.de\/wp-content\/uploads\/projekt-f06-bild02.png 1552w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><p id=\"caption-attachment-347\" class=\"wp-caption-text\">Cytokine expression of treated M2 macrophages. \u00a9Leonard Blum<\/p><\/div>\n<p><strong>Scientific goal: <\/strong><\/p>\n<p>As part of the project, a safety and\/or immunomodulatory profile will be created for small molecules\/peptides that have a potential antiviral, antiparasitic or antibacterial property. Furthermore, human proteins (e.g. TMPRSS2) which represent new targets for combating pathogens will be investigated.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>DRUID Collaboration partners<\/strong><strong>:<\/strong><\/p>\n<p>A1 Becker lab, A2 Gr\u00fcnweller lab, A3 Weber lab, B1 Diederich\/Kolb lab, B3 Rahlfs\/Kolb\/van Zandbergen, B5 Schlitzer lab, D1 Friebertsh\u00e4user lab<\/p>\n<hr \/>\n<p>References <strong>E6: 1. <\/strong>Blum et al.<strong> (2021) J Mol Med <\/strong>99(2):261-72;<strong> 2. <\/strong>Blum et al.<strong> (2020) Sci Rep <\/strong>10(1):7534; <strong>3. <\/strong>Blum et al.<strong> (2020) J Cell Mol Med <\/strong>24(12):6988-99.<\/p>\n<\/div>            <\/div>        <\/div>    <\/div><\/section><section class=\"av_toggle_section\" >    <div role=\"tablist\" class=\"single_toggle\" data-tags=\"{Alle}\">        <p data-fake-id=\"#toggle-id-1\" class=\"toggler av-inherit-border-color\" role=\"tab\" tabindex=\"0\" aria-controls=\"1toggle-id-container\"><span class=\"project_nr\"> E7 P<\/span><span class=\"project_title\">Neglected infectious diseases with focus on imaging<\/span><br><span class=\"project_names\">Jacomina Krijnse Locker<\/span><span class=\"toggle_icon\">        <span class=\"vert_icon\"><\/span><span class=\"hor_icon\"><\/span><\/span><\/p>        <div id=\"toggle-id-1-container\" class=\"toggle_wrap\">            <div class=\"toggle_content invers-color invers-color  av-inherit-border-color flex-container\">              <div class=\"projekt-names one_three\"><div class=\"project-name\"><div class=\"flex-container\"><div class=\"half\"><span class=\"principal\">Principal Investigator:<\/span><\/div><div class=\"half\"><a href=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/jacomina-krijnse-locker.jpg\"><img decoding=\"async\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/pls2022\/jacomina-krijnse-locker.jpg\" \/><\/a><\/div><\/div><p class=\"person_name\"><strong>Prof. Dr. Jacomina Krijnse Locker<\/strong><\/p><p><p>Elektronenmikroskopie von Pathogenen<br \/>\nPaul-Ehrlich-Institut<br \/>\nPaul-Ehrlich-Stra\u00dfe 51-59<br \/>\n63225 Langen<br \/>\nTel.: +49 (0)6103-77 2011<br \/>\nE-Mail: <a class=\"mail\" href=\"mailto:Jacomina.KrijnseLocker@pei.de\">Jacomina.KrijnseLocker(at)pei(dot)de<\/a><\/p>\n<\/p><\/div><hr><\/div>\t\t\t <div class=\"project-content two_three border-left\"><p><strong>Project description:<\/strong><\/p>\n<p>We apply imaging techniques to understand how pathogens interact with cells to cause disease, focusing on electron microscopy (EM) and correlated light- and electron microscopy (CLEM). To generate robust protocols that can easily be adapted, we use our model virus, the large DNA-virus vaccinia (VACV).<\/p>\n<p>The SARS CoV2 pandemic illustrates how viruses have a world-wide socio-economical impact with dramatic consequences for the neglected tropical diseases (NTDs). There is an urgent need to contain the SARS CoV2 pandemic and refocus resources on NTDs. In a collaborative effort we showed that the spike-surface protein (S) of SARS COV2, that mediates infection, shows unexpected flexibility. The latter could facilitate receptor binding and cell entry of the virus, which remains to demonstrated.<\/p>\n<div id=\"attachment_402\" style=\"width: 179px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-402\" class=\"wp-image-402 size-full\" src=\"https:\/\/www.loewe-druid.de\/wp-content\/uploads\/projekt-c07p-bild01.gif\" alt=\"\" width=\"169\" height=\"109\" \/><p id=\"caption-attachment-402\" class=\"wp-caption-text\">Cryo-electron tomography and molecular dynamic simulation of SARS-CoV-2 spike shows unexpected flexibility of the stalk.<\/p><\/div>\n<p>Upon budding at intracellular membranes coronaviruses leave the cell in a poorly explored way. Vesicular trafficking as well as regulated lysosomal exocytosis have been proposed.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>Scientific goal:<\/strong><\/p>\n<p>The project aims to interfere with the flexibility of S and test for the outcome of infection using antibodies, drugs and genetics. Virus release from infected cells will be studied by a CLEM approach, combining live cell imaging with EM.\u00a0 The expertise gained from our VACV- and SARS CoV2 host system is then applied to answer questions related to virus-host systems used in other teams of the DRUID-consortium.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>DRUID Collaboration partners<\/strong><strong>:<\/strong><\/p>\n<p>A3 Weber lab, B2 Ziebuhr lab, B6 P Herker lab, C1 Hildt lab, D3 van Zandbergen lab, E5 Czermak lab<\/p>\n<hr \/>\n<p>References E7 P: Turo\u0148ov\u00e1, B., et al. <strong>(2020).<\/strong> <strong><em>Science<\/em><\/strong> (80-. ). <em>370<\/em>, 203\u2013208. Blanco-Rodriguez, G., et al<strong>. (2020)<\/strong>. <strong><em>J. Virol<\/em><\/strong>. <em>94<\/em> e00135-20. Quemin, E.R.,\u00a0 et al. <strong>(2018).<\/strong> <strong><em>J. Mol. <\/em><\/strong><strong><em>Biol.<\/em><\/strong><em>430, <\/em>1714-1724. Chlanda, P., and Krijnse Locker, J. <strong>(2017).<\/strong> <strong><em>Biochem. J<\/em><\/strong>. <em>474<\/em>, 1041\u20131053. Sartori-Rupp, A., et al. <strong>(2019)<\/strong>. <strong><em>Nat. Commun<\/em><\/strong>. <em>10<\/em>, 342.<\/p>\n<\/div>            <\/div>        <\/div>    <\/div><\/section><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"parent":140,"menu_order":13,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-150","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.2 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Project area E - LOEWE-Zentrum DRUID<\/title>\n<meta name=\"description\" content=\"Find here detailed information Project area E of LOEWE-DRUID\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.loewe-druid.de\/en\/research\/project-area-e\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Project area E - 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